Use of high throughput amplicon sequencing and ethidium monoazide dye to track microbiota changes in an equol-producing menopausal woman receiving a long-term isoflavones treatment
Use of high throughput amplicon sequencing and ethidium monoazide dye to track microbiota changes in an equol-producing menopausal woman receiving a long-term isoflavones treatment
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DOI：
10.3934/microbiol.2019.1.102
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发表时间：
2019-01-01
影响因子：
4.8
通讯作者：
Delgado, Susana
中科院分区：
文献类型：
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作者：
Guadamuro, Lucia;Andrea Azcarate-Peril, M.;Delgado, Susana
这项工作描述了长期食用富含雌马酚的膳食每日补充剂对绝经期雌马酚生产妇女粪便微生物群的组成和多样性的影响。对处理0、1、3和6个月时采集的粪便样本进行16S rDNA扩增子测序。此外，出于比较目的，使用单叠氮乙锭（EMA）以避免检测到来自死亡细菌的DNA。两个属的家庭Coriobacteriaceae（Eggerthella和Collinsella）的成员被发现在所有采样点在补充维生素C的比例更大。Ruminococcaceae（E.例如，在一个实施例中，瘤胃球菌属和粪杆菌属），以及毛螺菌科（粪球菌属）的成员也经历了显着增加。对于这最后一个属，发现与尿中雌马酚排泄水平呈正相关。目前已知参与代谢和产生雌马酚的细菌菌株已被分配到这些分类群。EMA染料的使用使我们能够解开这些细菌的肠道（e。例如，在一个实施例中，毛螺菌科），更容易受到损害。我们的研究有助于鉴定可能参与生产雌马酮所需代谢物（如雌马酚）的微生物，这些代谢物最终可以被分离出来，并被那些不能从雌马酮中自然获益的人进一步用作益生菌。
This work describes the impact of long term consumption of an isoflavone-rich dietary daily supplement on the composition and diversity of the faecal microbiota of a menopausal, equol-producing woman. Sequencing of 16S rDNA amplicons was performed on faecal samples taken at 0, 1, 3 and 6 months of treatment. Additionally, and for comparative purposes, ethidium monoazide (EMA) was used to avoid detection of DNA from dead bacteria. Members of two genera of the family Coriobacteriaceae (Eggerthella and Collinsella) were found in greater proportions at all sampling points during isoflavone supplementation. Different genera of the family Ruminococcaceae (e. g., Ruminococcus and Faecalibacterium), as well as members of the family Lachnospiraceae (Coprococcus) also underwent significant increases. For this last genus a positive correlation with the levels of equol excretion in urine was found. Currently bacterial strains known to be involved in isoflavone metabolism and equol production have been assigned to these taxa. The use of EMA dye allowed us to unravel those bacterial gut linages (e. g., Lachnospiraceae) that were more susceptible to damage. Our study contributes to the identification of microorganisms possibly involved in the production of isoflavone-desirable metabolites (such as equol), which could ultimately be isolated and further used as probiotics by people who cannot naturally benefit from isoflavones.