Photosynthetic production of itaconic acid in Synechocystis sp. PCC6803.
Photosynthetic production of itaconic acid in Synechocystis sp. PCC6803.
复制标题
DOI：
10.1016/j.jbiotec.2014.12.016
复制
发表时间：
2015-02
影响因子：
4.1
通讯作者：
T. Chin;M. Sano;Tetsuya Takahashi;H. Ohara;Y. Aso
中科院分区：
文献类型：
--
作者：
T. Chin;M. Sano;Tetsuya Takahashi;H. Ohara;Y. Aso
在这里，我们报道了聚球藻从二氧化碳(CO2)中光合作用产生衣康酸(IA)的过程，衣康酸是一种很有前途的建筑材料。PCC6803。表达顺式乌头酸脱羧酶的工程菌PCC6803在空气和5%二氧化碳持续鼓泡的条件下，分别以42.8g/μL−1day−和919.0μg/L−1d−1的速率产IA，产量分别为0.9g/L和14.5g/L。这是关于蓝藻通过光合作用从二氧化碳中产生IA的可能性的第一个报告。
Here, we report the photosynthetic production of itaconic acid (IA), a promising building block, from carbon dioxide (CO2) bySynechocystissp. PCC6803. The engineered PCC6803 strain expressingcis-aconitate decarboxylase, the key enzyme in IA biosynthesis, produced 0.9 mg/L and 14.5 mg/L of IA at production rates of 42.8 μg L−1day−1and 919.0 μg L−1day−1, under conditions of constant bubbling with air and 5% CO2, respectively. This is the first report on the possibility of IA production from CO2via the photosynthetic process in cyanobacteria.