HEPATOCYTE GROWTH-FACTOR ACCELERATES RECOVERY FROM ACUTE ISCHEMIC RENAL INJURY IN RATS
HEPATOCYTE GROWTH-FACTOR ACCELERATES RECOVERY FROM ACUTE ISCHEMIC RENAL INJURY IN RATS
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DOI：
10.1152/ajprenal.1994.266.1.f129
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发表时间：
1994-01-01
影响因子：
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通讯作者：
HAMMERMAN, MR
中科院分区：
文献类型：
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作者：
MILLER, SB;MARTIN, DR;HAMMERMAN, MR
在大鼠双侧肾动脉阻塞引起的急性缺血性肾损伤模型中检查了肝细胞生长因子（HGF）给药的作用。与给予载体的大鼠相比，缺血后30分钟皮下给予20 μ g HGF的大鼠在闭塞后7天的过程中血清肌酐和血尿素氮水平显著降低，在闭塞后第2天测量的菊粉清除率增强，死亡率降低，并且损伤后7天通过肾组织学检查明显减少。与未损伤大鼠肾脏中的掺入相比，皮质肾小管上皮中5-溴-2 '-脱氧尿苷（BrdU）的掺入增加反映了缺血后损伤发生的肾小管再生。与载体相比，HGF增强BrdU掺入，表明增强的有丝分裂。我们使用的HGF剂量不能改善缺血后损伤发生的体重减轻。我们的结论是，肝细胞生长因子缺血后损伤大鼠刺激正常肾功能的恢复和近端肾小管上皮细胞的再生。
Effects of hepatocyte growth factor (HGF) administration were examined in a model of acute ischemic renal injury induced by bilateral renal artery occlusion in rats. Compared with rats administered vehicle, rats administered 20 mu g HGF subcutaneously 30 min postischemia had significantly lower serum creatinine and blood urea nitrogen levels over the course of 7 days postocclusion, enhanced inulin clearances measured on day 2 postocclusion, reduced mortality, and much less injury evident by examination of kidney histologies 7 days postinjury. The tubular regeneration that occurred postischemic injury was reflected by increased incorporation of 5-bromo-2'-deoxyuridine (BrdU) in cortical tubular epithelium compared with incorporation in kidneys from noninjured rats. HGF enhanced BrdU incorporation compared with vehicle, indicating enhanced mitogenesis. The weight loss that occurs postischemic injury was not ameliorated by the dose of HGF we employed. We conclude that administration of HGF postischemic injury to rats stimulates the recovery of normal kidney function and the regeneration of proximal tubular epithelium.