Prostaglandin D2 production and identification of prostaglandin H synthase within canine mast cell granule.
Prostaglandin D2 production and identification of prostaglandin H synthase within canine mast cell granule.
复制标题
犬肥大细胞颗粒内前列腺素 D2 的产生和前列腺素 H 合酶的鉴定。
DOI：
10.1152/ajplung.1992.263.2.l168
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Lazarus,SC
中科院分区：
文献类型：
--
作者：
Thomas,PS;Wilson,AN;Schreck,RE;Lazarus,SC
我们已经确定了功能性前列腺素H合酶（PGH合酶，EC）的存在1.14.99.1这证实了颗粒内存在酶和底物。PGH合酶的颗粒的本地化，证实了通过免疫印迹的纯颗粒制剂，并通过免疫细胞化学使用的全细胞。在功能研究中，秋水仙碱，一种微管聚合抑制剂，导致组胺释放量和PGD2产生量下降高达70%。这表明功能性PGH合酶与颗粒密切相关并共同激活，或者该酶与微管系统有独立的关联。肥大细胞的预先形成的和新形成的介质的释放似乎是密切相关的，因此，防止脱粒可以减弱这两类介质的作用。
We have identified the presence of functional prostaglandin H synthase (PGH synthase, E.C. 1.14.99.1, or cyclooxygenase) within canine mast cell granules by demonstrating the generation of prostaglandin (PG) D2 from isolated and purified granules incubated with substrate as arachidonic acid or stimulated with calcium ionophore, A23187. This confirms the presence of both enzyme and substrate within the granule. Localization of PGH synthase to the granule was confirmed by immunoblotting of the pure granule preparation and by immunocytochemistry using the whole cell. In functional studies, colchicine, a microtubule polymerization inhibitor, caused a fall of up to 70%, both in the amount of histamine released and in the amount of PGD2 generated. This suggests either that functional PGH synthase is closely associated and coactivated with granules or that there is an independent association of this enzyme with the microtubule system. Release of the preformed and newly formed mediators of the mast cell appear to be closely linked, and prevention of degranulation may therefore attenuate the effects of both classes of mediators.