Production of CD4⁺ and CD8⁺ T cell hybridomas.
Production of CD4⁺ and CD8⁺ T cell hybridomas.
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DOI：
10.1007/978-1-62703-218-6_22
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发表时间：
2013-01-01
期刊：
影响因子：
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通讯作者：
Canaday, David H
中科院分区：
文献类型：
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作者：
Canaday, David H
T细胞杂交瘤是研究抗原提呈细胞(APC)功能的重要工具。它们是在融合技术的基础上开发出来的，融合技术导致了单抗切片。产生抗原特异性的初级T细胞，并将其融合到一个不朽的胸腺瘤系中。未融合的胸腺瘤细胞通过工程代谢选择被消除。抗原特异性杂交瘤已被鉴定，并可能被详细鉴定。原代T细胞是研究T细胞内在调节机制的首选细胞，但在抗原递呈研究中，T细胞杂交瘤比原始T细胞克隆具有优势，包括它们的相对一致性、随时间的稳定性以及大量可用于广泛的抗原递呈实验。
T cell hybridomas are very useful tools to investigate antigen presenting cell (APC) function. They were developed based on the fusion technology that led to monoclonal antibody section. Antigen-specific primary T cells are generated and fused to an immortal thymoma line. Unfused thymoma cells are eliminated by engineered metabolic selection. Antigen-specific hybridomas are identified and may be characterized in detail. Primary T cells are preferable for studies of the regulatory mechanisms intrinsic to T cells, but for study of antigen presentation T cell hybridomas have advantages over primary T cell clones, including their relative uniformity, stability over time, and ready availability in large numbers for extensive antigen presentation experiments.