Epidermal-skin-test 1000 (EST-1000) - A new reconstructed epidermis for in vitro skin corrosivity testing
Epidermal-skin-test 1000 (EST-1000) - A new reconstructed epidermis for in vitro skin corrosivity testing
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DOI：
10.1016/j.tiv.2005.06.010
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发表时间：
2005-10-01
影响因子：
3.2
通讯作者：
Fuchs, HW
中科院分区：
文献类型：
--
作者：
Hoffmann, J;Heisler, E;Fuchs, HW
确定某些产品和成分的潜在腐蚀性或刺激性对其分类和标签要求是必要的。重建皮肤作为一个模型系统为单细胞培养测试提供了基本的优势，并在不同的验证研究中显示出有希望的结果（回顾：Fentem， j.h., Botham, p.a., 2002）。ECVAM在验证皮肤腐蚀和刺激替代测试方面的活动。ATLA 30(增刊。2), 61 - 67)。在本研究中，我们介绍了我们的新重建表皮“表皮-皮肤测试”（EST-1000）。完全生长的表皮由增殖和分化的角质形成细胞组成。组织学和免疫组织化学数据显示，EST-1000与正常人皮肤具有高度的可比性。特征标记（KI-67、ck1/10/5 /14、转谷氨酰胺酶、IV型胶原蛋白、天青蛋白、β 1整合素）易于识别。这项工作的主要重点是通过测试几种已知具有腐蚀性的物质来表征EST-1000，特别是其屏障功能。皮肤腐蚀是通过短期应用于角质层后对重建表皮的细胞毒性作用来检测的。通过标准MTT试验和伴随的组织学分析来确定效果。因此，EST-1000显示出非常高的预测潜力，并缩小了动物试验与已建立的全层模型Advanced-Skin-Test 2000 （AST-2000）之间的差距。， Kandsberger， M, Matthes， T., Fuchs， H., Graeve， T., 1999。重建人体皮肤（AST-2000）作为药物毒理学工具。Atla 27,302)。(c) 2005 Elsevier Ltd版权所有。
The determination of a possible corrosive or irritative potential of certain products and ingredients is necessary for their classification and labeling requirements. Reconstructed skin as a model system provides fundamental advantages to single cell culture testing and leads to promising results as shown by different validation studies (for review: Fentem, J.H., Botham, P.A., 2002. ECVAM's activities in validating alternative tests for skin corrosion and irritation. ATLA 30(Suppl. 2), 61-67). In this study we introduce our new reconstructed epidermis "Epidermal-Skin-Test" (EST-1000). This fully grown epidermis consists of proliferating as well as differentiating keratinocytes. EST-1000 shows a high comparability to normal human skin as shown by histological and immuno-histochemical data. Characteristic markers (KI-67, CK 1/10/5/14, transglutaminase, collagen IV, involucrin, beta 1 integrin) can be identified easily. The main focus of this work was to characterize EST-1000 especially with respect to its barrier function by testing several substances of known corrosive potential. Skin corrosion was detected by the cytotoxic effect of the substances on a reconstructed epidermis after short-term application to the stratum corneum. The effect was determined by standard MTT assay and accompanying histological analysis. Hence EST-1000 shows a very high predictive potential and closes the gap between animal testing and the established full-thickness model Advanced-Skin-Test 2000 (AST-2000) (Noll, M., Merkle, M.-L., Kandsberger, M., Matthes, T., Fuchs, H., Graeve, T., 1999. Reconstructed human skin (AST-2000) as a tool for pharmaco-toxicology. ATLA 27, 302). (c) 2005 Elsevier Ltd. All rights reserved.