Genome-wide identification and comparative analysis of grafting-responsive mRNA in watermelon grafted onto bottle gourd and squash rootstocks by high-throughput sequencing
Genome-wide identification and comparative analysis of grafting-responsive mRNA in watermelon grafted onto bottle gourd and squash rootstocks by high-throughput sequencing
复制标题
高通量测序对葫芦和南瓜砧木西瓜嫁接反应 mRNA 的全基因组鉴定和比较分析
DOI：
10.1007/s00438-015-1132-5
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发表时间：
2016-04-01
影响因子：
3.1
通讯作者：
Zhang, Mingfang
中科院分区：
文献类型：
--
作者：
Liu, Na;Yang, Jinghua;Zhang, Mingfang
嫁接是一种重要的农业技术，广泛用于改善植物生长、产量和对生物或非生物胁迫的适应。然而，嫁接诱导的生理过程的分子机制仍不清楚。西瓜（Citrullus lanatus L.）是世界范围内重要的园艺作物。嫁接技术常用于西瓜生产，以提高其对胁迫的耐受性，特别是对土传镰刀菌枯萎病的耐受性。在本研究中，我们使用高通量测序对嫁接到葫芦和南瓜砧木上的西瓜接穗进行全基因组转录本分析。我们的转录组和数字基因表达（DGE）分析数据提供了对嫁接西瓜基因调控分子方面的见解。与自嫁接西瓜相比，葫芦和西葫芦砧木嫁接西瓜分别有787个和3485个差异表达基因。这些基因与初级和次级代谢、激素信号、转录因子、转运蛋白和对刺激的反应有关。嫁接导致这些基因表达的变化，表明它们可能在介导嫁接苗的生理过程中发挥重要作用。讨论了嫁接响应 mRNA 在多种生物和代谢过程中的潜在作用。显然，本研究获得的数据为揭示候选基因在不同生物过程和移植系统环境适应中的功能机制提供了极好的资源。
Grafting is an important agricultural technique widely used to improve plant growth, yield, and adaptation to either biotic or abiotic stresses. However, the molecular mechanisms underlying grafting-induced physiological processes remain unclear. Watermelon (Citrullus lanatus L.) is an important horticultural crop worldwide. Grafting technique is commonly used in watermelon production for improving its tolerance to stresses, especially to the soil-borne fusarium wilt disease. In the present study, we used high-throughput sequencing to perform a genome-wide transcript analysis of scions from watermelon grafted onto bottle gourd and squash rootstocks. Our transcriptome and digital gene expression (DGE) profiling data provided insights into the molecular aspects of gene regulation in grafted watermelon. Compared with self-grafted watermelon, there were 787 and 3485 genes differentially expressed in watermelon grafted onto bottle gourd and squash rootstocks, respectively. These genes were associated with primary and secondary metabolism, hormone signaling, transcription factors, transporters, and response to stimuli. Grafting led to changes in expression of these genes, suggesting that they may play important roles in mediating the physiological processes of grafted seedlings. The potential roles of the grafting-responsive mRNAs in diverse biological and metabolic processes were discussed. Obviously, the data obtained in this study provide an excellent resource for unraveling the mechanisms of candidate genes function in diverse biological processes and in environmental adaptation in a graft system.