Vascular endothelial-junctional adhesion molecule (VE-JAM)/JAM 2 interacts with T, NK, and dendritic cells through JAM 3
Vascular endothelial-junctional adhesion molecule (VE-JAM)/JAM 2 interacts with T, NK, and dendritic cells through JAM 3
复制标题
DOI：
10.4049/jimmunol.168.4.1618
复制
发表时间：
2002-02-15
影响因子：
4.4
通讯作者：
Fong, S
中科院分区：
文献类型：
--
作者：
Liang, TW;Chiu, HH;Fong, S
筛选表达的序列标签数据库的内皮特异性同源物的人连接粘附分子（JAM）和A33-Ag，我们确定了一个蛋白质298作为代表最近描述的血管内皮-JAM（VE-JAM）/JAM 2。我们证实VE-JAM/JAM 2表达仅限于扁桃体和淋巴结的高内皮微静脉，并且我们进一步扩大了在炎症和肿瘤病灶内和周围的小动脉内皮的定位。在我们对VE-JAM/JAM 2的功能表征中，我们发现它可以作为T细胞系J 45的粘附配体起作用，并且可以与GM-CSF/IL-4衍生的外周血树突状细胞、循环CD 56（+）NK细胞、循环CD 56（+）CD 3（+）NK/T细胞和循环CD 56（+）CD 3（+）CD 8（+）细胞溶解性T细胞相互作用。在我们的研究过程中，我们还分离并表征了功能性VE-JAM/JAM 2受体，克隆后，其被证明是代表JAM 3的提交序列（登录号NP 113658）。有了这些理解，我们已经确定了一个蛋白质相互作用对，它在T，NK和树突状细胞运输和炎症中的作用可能是重要的。
Screening expressed sequence tag databases for endothelial-specific homologs to human junctional adhesion molecule (JAM) and A33-Ag, we identified a protein of 298 as that represents the recently described vascular endothelial-JAM (VE-JAM)/JAM 2. We confirmed VE-JAM/JAM 2 expression to be restricted to the high endothelial venule of tonsil and lymph nodes, and we further expanded the localization to the endothelium of arterioles in and around inflammatory and tumor foci. In our functional characterizations of VE-JAM/JAM 2, we discovered that it can function as an adhesive ligand for the T cell line J45 and can interact with GM-CSF/IL-4-derived peripheral blood dendritic cells, circulating CD56(+) NK cells, circulating CD56(+)CD3(+) NK/T cells, and circulating CD56(+)CD3(+)CD8(+) cytolytic T cells. In the course of our studies, we also isolated and characterized the functional VE-JAM/JAM 2 receptor, which, upon cloning, turned out to be a submitted sequence representing JAM 3 (accession number NP 113658). With these understandings, we have characterized a protein-interacting pair that can be important in the role of T, NK, and dendritic cell trafficking and inflammation.