FXPRL-amide peptides induce ecdysteroidogenesis through a G-protein coupled receptor expressed in the prothoracic gland of Bombyx mori
FXPRL-amide peptides induce ecdysteroidogenesis through a G-protein coupled receptor expressed in the prothoracic gland of Bombyx mori
复制标题
DOI：
10.1016/j.mce.2007.05.008
复制
发表时间：
2007-07
影响因子：
4.1
通讯作者：
Ken Watanabe;J. Hull;T. Niimi;K. Imai;S. Matsumoto;T. Yaginuma;H. Kataoka
中科院分区：
文献类型：
--
作者：
Ken Watanabe;J. Hull;T. Niimi;K. Imai;S. Matsumoto;T. Yaginuma;H. Kataoka
FXPRL-酰胺肽家族（焦激肽/PBAN家族）由在昆虫生命过程中广泛发挥功能的昆虫肽组成，并且以保守的C-末端基序为特征。在家蚕中，性信息素的生物合成和胚胎滞育的诱导都受到来自该家族的肽的调节。为了阐明FXPRL酰胺肽的其他功能，我们分析了这些肽的两种已知的FXPRL G蛋白偶联受体的组织表达模式。研究发现，家蚕滞育激素受体（BmDHR）在前胸腺（PG）中表达，前胸腺是合成和释放蜕皮激素的器官。此外，滞育激素（DH），一个成员的FXR-酰胺肽，增加细胞内Ca 2+和cAMP浓度，并诱导蜕皮甾体激素在晚五龄PG与BmDHR表达的PG一致。在FXPRL-酰胺肽中，DH也具有最高的prothoracotropic活性，这很好地对应于异源表达的BmDHR的配体特异性。这些结果表明，FXPRL-酰胺肽可以作为prothoracotropic因子通过激活BmDHR，并可能在控制蜕皮和变态中发挥重要作用。
The FXPRL-amide peptide family (pyrokinin/PBAN family) consists of insect peptides that function broadly in insect life processes and are characterized by a conserved C-terminal motif. In the silkworm, Bombyx mori, sex pheromone biosynthesis and induction of embryonic diapause are regulated by peptides from this family. To elucidate other functions of Bombyx FXPRL-amide peptides, we analyzed the tissue expression patterns of two known Bombyx G-protein coupled receptors for these peptides. We found that the Bombyx diapause hormone receptor (BmDHR), is expressed in the prothoracic gland (PG), the organ which synthesizes and releases the insect molting hormones, ecdysteroids. Furthermore, diapause hormone (DH), a member of the Bombyx FXPRL-amide peptides, increases both intracellular Ca2+and cAMP concentrations and induces ecdysteroidogenesis in late fifth instar PGs coincident with BmDHR expression in the PGs. DH also has the highest prothoracicotropic activity among the FXPRL-amide peptides, which corresponds well to the ligand specificity of heterologously expressed BmDHR. These results demonstrate that FXPRL-amide peptides can function as prothoracicotropic factors through the activation of BmDHR and may play an important role in controlling molting and metamorphosis.