WIDESPREAD EXPRESSION OF GLYCINE RECEPTOR SUBUNIT MESSENGER-RNAS IN THE ADULT AND DEVELOPING RAT-BRAIN
WIDESPREAD EXPRESSION OF GLYCINE RECEPTOR SUBUNIT MESSENGER-RNAS IN THE ADULT AND DEVELOPING RAT-BRAIN
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DOI：
10.1002/j.1460-2075.1991.tb07779.x
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发表时间：
1991-09-01
期刊：
影响因子：
11.4
通讯作者：
BETZ, H
中科院分区：
文献类型：
--
作者：
MALOSIO, ML;MARQUEZEPOUEY, B;BETZ, H
抑制性甘氨酸受体 (GlyR) 是一种配体门控离子通道，介导脊髓和脊椎动物中枢神经系统其他区域的突触后抑制。先前的生化和分子克隆研究表明 GlyR 在发育过程中存在异质性。在这里，使用序列特异性寡核苷酸探针通过原位杂交研究了 GlyR 亚基转录物在大鼠脑和脊髓中的分布。在成年动物中，GlyR α-1 亚基 mRNA 在脊髓中含量丰富，但在一些大脑区域也可见，例如大脑区域。上丘和下丘，而 alpha-2 转录物在多个大脑区域中发现，包括大脑皮层第六层和海马体。 GlyR α-3 亚基 mRNA 在小脑、嗅球和海马中低水平表达，而大量 β 亚基转录物广泛分布在整个脊髓和大脑中。在发育过程中，α-2 mRNA 在产前就已经积累并在出生后减少，而 α-1 和 α-3 亚基转录物仅出现在出生后的大脑结构中。 β亚基 mRNA 的杂交信号在早期胚胎阶段就已可见，并在成年大鼠中持续增加至高水平。这些数据揭示了 GlyR 亚基 mRNA 的区域和发育表达的意外差异，并指出了 GlyR 蛋白在哺乳动物中枢神经系统中的新功能。
The inhibitory glycine receptor (GlyR) is a ligand-gated ion channel which mediates post-synaptic inhibition in spinal cord and other regions of the vertebrate central nervous system. Previous biochemical and molecular cloning studies have indicated heterogeneity of GlyRs during development. Here, the distribution of GlyR subunit transcripts in rat brain and spinal cord was investigated by in situ hybridization using sequence-specific oligonucleotide probes. In adult animals, GlyR alpha-1 subunit mRNA was abundant in spinal cord, but was also seen in a few brain areas, e.g. superior and inferior colliculi, whereas alpha-2 transcripts were found in several brain regions including layer VI of the cerebral cortex and hippocampus. GlyR alpha-3 subunit mRNA was expressed at low levels in cerebellum, olfactory bulb and hippocampus, while high amounts of beta-subunit transcripts were widely distributed throughout spinal cord and brain. During development, alpha-2 mRNA accumulated already prenatally and decreased after birth, whereas alpha-1 and alpha-3 subunit transcripts appeared only in postnatal brain structures. Hybridization signals of beta-subunit mRNA were seen already at early embryonic stages and continuously increased to high levels in adult rats. These data reveal unexpected differences in the regional and developmental expression of GlyR subunit mRNAs and point to novel functions of GlyR proteins in the mammalian central nervous system.