Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9 .3. Specificity of the in vitro antigen-specific gamma interferon test for bovine brucellosis diagnosis in experimentally Yersinia enterocolitica 0:9-infected cattle
Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9 .3. Specificity of the in vitro antigen-specific gamma interferon test for bovine brucellosis diagnosis in experimentally Yersinia enterocolitica 0:9-infected cattle
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DOI：
10.1016/s0378-1135(97)00110-7
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发表时间：
1997-10-16
影响因子：
3.3
通讯作者：
Staak, C
中科院分区：
文献类型：
--
作者：
Kittelberger, R;Reichel, MP;Staak, C
采用常规的布鲁氏菌病补体结合试验（CFT）、血清凝集试验（SAT）、布鲁氏菌卡片试验（BCT）和新近开发的布鲁氏菌抗原特异性γ干扰素（IFN-γ）试验，对10头小肠结肠炎耶尔森菌（Yersinia enterocolitica，0：9）实验感染母牛的免疫反应过程进行了跟踪。最初，动物经口暴露于10个菌落形成单位（CFU）的Y。小肠结肠炎0：9。4周后，静脉接种10（8）CFU的Y。小肠结肠炎0：9细胞。经口接种后，在常规布鲁氏菌病试验中的反应极小。只有在静脉接种后，CFT和SAT滴度以及BCT反应才与自然的假阳性布鲁氏菌病反应者相当。经口暴露后，80%的小母牛的布鲁氏菌素刺激IFN-γ释放达到峰值，高于临界刺激指数2.5。静脉接种后，仅10%的动物的刺激指数高于2.5。两个B。流产感染的对照牛显示刺激指数为3.1和3.4，阴性对照动物显示刺激指数为1.0。这些结果表明，与先前的研究相反，布鲁氏菌特异性IFN-γ检测不能用作B的特异性和区分性检测。流产感染(C)1997年Elsevier Science B.V.
The course of immunological reaction in 10 Yersinia enterocolitica 0:9 experimentally-infected heifers was followed using the conventional brucellosis tests complement fixation test (CFT), serum agglutination test (SAT) and brucella card test (BCT), and a recently developed Brucella antigen-specific gamma interferon (IFN-gamma) test. Initially, the animals were exposed orally to 10(10) colony-forming units (CFU) of Y. enterocolitica 0:9. Four weeks later, they were inoculated intravenously with 10(8) CFU of Y. enterocolitica 0:9 cells. After oral inoculation, the response in the conventional brucellosis tests was minimal. Only after intravenous inoculation were CFT and SAT titres and BCT reactions comparable to natural, false positive brucellosis reactors. After oral exposure the Brucellergen-stimulated release of IFN-gamma peaked at values above the cut-off stimulation index of 2.5 in 80% of the heifers, After intravenous inoculation, stimulation indices above 2.5 were present in only 10% of the animals. Two B. abortus infected control cattle showed stimulation indices of 3.1 and 3.4, and a negative control animal exhibited a stimulation index of 1.0. These findings show, in contrast to a previous study, that the Brucellergen-specific IFN-gamma assay cannot be used as a specific and discriminatory test for B. abortus infections. (C) 1997 Elsevier Science B.V.