Expression of Notch receptors and ligands in the adult gut
Expression of Notch receptors and ligands in the adult gut
复制标题
DOI：
10.1177/002215540405200409
复制
发表时间：
2004-04-01
影响因子：
3.2
通讯作者：
Powell, BC
中科院分区：
文献类型：
--
作者：
Sander, GR;Powell, BC
Notch信号通路已被认为是调控细胞增殖/分化决策和细胞命运的重要途径。为了探讨Notch通路在成人肠道中的作用，我们用Northern印迹和原位杂交的方法研究了Notch受体及其配体的表达。Notch受体和配体在成年大鼠肠道的增殖细胞和有丝分裂后细胞中都有表达，在上皮细胞、免疫细胞和内皮细胞中表达不同。Notch1、Jagged1和Jagged2的表达经常重叠，而Notch2的表达仅限于特定的隐窝细胞、大肠固有层和Peyer‘s斑块淋巴细胞。我们认为，多种Notch受体和配体在一系列不同类型的肠道细胞中的表达表明，这一信号通路支持许多涉及成人肠道维持和功能的过程。
The Notch signaling pathway has become recognized as a vitally important pathway in regulating proliferative/differentiative decisions and cell fate. To explore the involvement of the Notch pathway in adult gut, we investigated the expression of Notch receptors and their ligands by Northern blotting and in situ hybridization. Notch receptors and ligands were expressed in both proliferative and post-mitotic cells throughout adult rat gut, variously in epithelial, immune, and endothelial cells. Expression of Notch1, Jagged1, and Jagged2 frequently overlapped, whereas Notch2 expression was restricted to specific crypt cells, the lamina propria of the large intestine, and Peyer's patch lymphocytes. We propose that the expression of multiple Notch receptors and ligands in a range of different intestinal cell types indicates that this signaling pathway underpins many of the processes involved in the maintenance and function of the adult gut.