Local T/B cooperation in inflamed tissues is supported by T follicular helper-like cells.
Local T/B cooperation in inflamed tissues is supported by T follicular helper-like cells.
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DOI：
10.1038/ncomms10875
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发表时间：
2016-02-26
影响因子：
16.6
通讯作者：
Hutloff A
中科院分区：
文献类型：
--
作者：
Vu Van D;Beier KC;Pietzke LJ;Al Baz MS;Feist RK;Gurka S;Hamelmann E;Kroczek RA;Hutloff A
自身免疫性疾病和其它炎性病症的特征在于大的淋巴细胞组织浸润，其中可以发现T和B细胞紧密接触。在此，我们使用小鼠气道炎症模型，比较了肺组织和肺引流淋巴结中抗原特异性T和B细胞。在肺中，我们确定了一个B细胞群体表现出经典的生发中心表型，而没有被组织成异位淋巴组织。相比之下，经典的CXCR 5 + Bcl-6+ T滤泡辅助细胞不存在。尽管如此，肺浸润T细胞表现出滤泡辅助细胞样特性，包括为幼稚B细胞提供帮助的潜力。肺组织也是记忆T和B细胞在炎症消退后残留在支气管周围浸润物中的生存小生境。总的来说，这项研究表明，T/B合作的重要性，不仅在淋巴结，而且在发炎的外周组织的局部抗体反应感染和自身免疫。 在次级淋巴器官中，T滤泡辅助（Tfh）细胞帮助B细胞发育成记忆B细胞和浆细胞。在这里，作者表明，发炎的肺成为具有生发中心表型的活化B细胞和尽管不表达经典Tfh标记物但表现出Tfh样特性的T细胞的储库。
Autoimmune diseases and other inflammatory conditions are characterized by large lymphocytic tissue infiltrates in which T and B cells can be found in close contact. Here, using a murine airway inflammation model, we compare antigen-specific T and B cells in lung tissue versus lung-draining lymph node. In the lung we identify a B-cell population exhibiting a classical germinal centre phenotype without being organized into ectopic lymphoid tissue. By contrast, classical CXCR5+ Bcl-6+ T follicular helper cells are not present. Nevertheless, lung-infiltrating T cells exhibit follicular helper-like properties including the potential to provide help to naive B cells. The lung tissue is also a survival niche for memory T and B cells remaining in residual peribronchial infiltrates after resolution of inflammation. Collectively, this study shows the importance of T/B cooperation not only in lymph nodes but also in inflamed peripheral tissues for local antibody responses to infection and autoimmunity. In secondary lymphoid organs T follicular helper (Tfh) cells help B cells to develop into memory B and plasma cells. Here, the authors show that inflamed lung becomes a reservoir of activated B cells with a germinal centre phenotype, and T cells that exhibit Tfh-like properties despite not expressing classical Tfh markers.