Differential expression of five sialyltransferase genes in human tissues.
Differential expression of five sialyltransferase genes in human tissues.
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发表时间：
1994-07
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通讯作者：
Hiroshi Kitagawa;J. C. Paulson
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作者：
Hiroshi Kitagawa;J. C. Paulson
细胞表面糖蛋白和糖脂上的特定碳水化合物结构的细胞类型特异性表达越来越多地被认为是提供与发育和成年生物体中的细胞-细胞相互作用相关的信息。唾液酸转移酶通过将唾液酸附着在糖脂和糖蛋白（N-连接和O-连接）碳水化合物基团上的不同末端位置而促进碳水化合物结构的多样性。在这份报告中，五个唾液酸转移酶基因在人体组织中的差异表达进行了评估，作为一个潜在的机制，占细胞类型特异性变化的终端唾液酸结构的细胞产生的。为了进行该分析，克隆了人Gal β 1，3GalNAc α 2，3-唾液酸转移酶的完整cDNA和发育调节STX基因的部分cDNA。用这些cDNA和以前克隆的三个人唾液酸转移酶基因的cDNA作为探针进行北方分析。五个唾液酸转移酶基因中的每一个都在16个成人和5个胎儿的人体组织中表现出显着的差异表达，每个基因的表达似乎是独立调节的。与早期对大鼠组织中几种相同酶表达的零碎研究相比，表明表达的总体模式在很大程度上是保守的。
Cell type-specific expression of specific carbohydrate structures on cell surface glycoproteins and glycolipids is increasingly recognized for providing information relevant to cell-cell interactions in developing and adult organisms. Sialyltransferases contribute to the diversity in carbohydrate structure through their attachment of sialic acid in various terminal positions on glycolipid and on glycoprotein (N-linked and O-linked) carbohydrate groups. In this report, differential expression of five sialyltransferase genes in human tissues is evaluated as a potential mechanism to account for cell type-specific variation in terminal sialoside structures produced by a cell. For this analysis, the complete cDNA of the human Gal beta 1,3GalNAc alpha 2,3-sialyltransferase and a partial cDNA of the developmentally regulated STX gene were cloned. Northern analysis was performed using these cDNAs and those of three previously cloned human sialyltransferase genes as probes. Each of the five sialyltransferase genes exhibits dramatic differential expression in the 16 adult and 5 fetal human tissues examined, and expression of each gene appears to be independently regulated. Comparison with fragmentary earlier studies of the expression of several of the same enzymes in rat tissues suggests that the overall pattern of expression is largely conserved.