DNA METHYLATION - CORRELATION WITH DNASE-I SENSITIVITY OF CHICKEN OVALBUMIN AND CONALBUMIN CHROMATIN
DNA METHYLATION - CORRELATION WITH DNASE-I SENSITIVITY OF CHICKEN OVALBUMIN AND CONALBUMIN CHROMATIN
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DOI：
10.1093/nar/7.8.2105
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发表时间：
1979-01-01
影响因子：
14.9
通讯作者：
CHAMBON, P
中科院分区：
文献类型：
--
作者：
KUO, MT;MANDEL, JL;CHAMBON, P
为了分析卵清蛋白和伴清蛋白基因区（1）某些位点的DNA甲基化不足与这些基因在鸡输卵管中的表达之间的关系，对从用DNA酶I处理的鸡红细胞或输卵管染色质中分离的DNA进行Hhal双酶切，Hhal可区分甲基化和非甲基化的Hhal限制性位点，DNA酶I优先降解“活性”染色质。随后用卵白蛋白和伴白蛋白特异性杂交探针进行分析。我们的结论是，在卵清蛋白和伴清蛋白基因区域1s的某些位点发现的残留DNA甲基化来自这些基因的染色质不处于“活性”形式的细胞部分。另一方面，卵清蛋白和伴清蛋白位点在红细胞DNA中部分未甲基化，对应于DNA酶I不敏感的染色质区域。我们还检测到伴清蛋白基因3′端下游约1kb处的一个位点，该位点在所有检测的组织中对DNase I高度敏感。
To analyse the relationship between DNA undermethylation at some sites in the ovalbumin and conalbumin gene regions (1) and the expression of these genes in chick oviduct, digestions with Hhal, which differentiates between methylated and unmethylated Hhal restriction sites, was performed on DNA isolated from chicken erythrocyte or oviduct chromatin treated with DNase I which degrades preferentially “active” chromatin. This was followed by analysis with ovalbumin- and conalbumin-specific hybridization probes. We conclude that the residual DNA methylation found at some sites of the ovalbumin and conalbumin gene regions 1s derived from the fraction of cells in which the chromatin of these genes is not in an “active” form. On the other hand, the ovalbumin and conalbumin sites which are partially unmethylated in erythrocyte DNA correspond to chromatin regions which are not DNase I-sensit1ve. We have also detected a site about 1 kb downstream from the 3′ end of the conalbumin gene that is hypersensitive to DNase I in all tissues tested.