Neuron-specific recombination by Cre recombinase inserted into the murine tau locus
Neuron-specific recombination by Cre recombinase inserted into the murine tau locus
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DOI：
10.1016/j.bbrc.2008.03.103
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发表时间：
2008-06-06
影响因子：
3.1
通讯作者：
Harada, Akihiro
中科院分区：
文献类型：
--
作者：
Muramatsu, Kazuhiro;Hashimoto, Yukiko;Harada, Akihiro
为了在体内确定基因的神经功能，需要在动物中对靶基因进行神经元特异性缺失。Tau是一种微管相关蛋白，在神经元中大量表达，但在胶质细胞和其他组织中几乎不表达。因此，为了产生在神经元中表达Cre重组酶的小鼠，我们将Cre重组酶插入tau基因座。通过将这些tau-Cre小鼠与ROSA 26 lacZ报告小鼠杂交，我们在来自大多数中枢神经系统的神经元中观察到Cre重组酶活性，但在胶质细胞和非神经元组织中均未观察到。这种神经元特异性活性出现在胚胎发生过程中。我们进一步将tau-Cre小鼠与rab 8“floxed”小鼠杂交，并显示重组在脑中几乎完全，但在其他组织中不完全或不可检测。因此，tau-Cre基因敲入小鼠是研究体内基因的神经元功能的有用工具。(c)2008年爱思唯尔公司All rights reserved.
To determine the neuronal function of genes in vivo, the neuron-specific deletion of a target gene in animals is required. Tau, a microtubule-associated protein, is expressed abundantly in neurons but scarcely in glias and other tissues. Therefore, to generate mice that express Cre recombinase in neurons, we inserted Cre recombinase into the tau locus. By crossing these tau-Cre mice with ROSA26 lacZ reporter mice, we observed Cre recombinase activity in the neurons from most of the central nervous system, but not in glias nor in non-neuronal tissues. This neuronal-specific activity appeared during embryogenesis. We further crossed tau-Cre mice with rab8 'floxed' mice, and showed that the recombination was nearly complete in the brain, but incomplete or non-detectable in other tissues. Thus, tau-Cre knockin mouse is a useful tool for studying the neuronal function of a gene in vivo. (c) 2008 Elsevier Inc. All rights reserved.