Production methods of virus particles
Production methods of virus particles
复制标题
病毒颗粒的生产方法
DOI：
10.1007/s10529-014-1741-9
复制
发表时间：
2015
影响因子：
2.7
通讯作者：
and Hiroaki Imataka
中科院分区：
文献类型：
--
作者：
Kodai Machida;and Hiroaki Imataka
病毒颗粒和病毒样颗粒（VLP）或衣壳正在成为生物成像、药物递送和材料科学中重要的载体和模板。病毒颗粒通过感染宿主生物体制备，但VLP从表达衣壳蛋白的细胞获得。一些VLP被拆卸，然后重新组装以包含感兴趣的材料。适合于操纵病毒组装过程的无细胞系统也可用于生产病毒颗粒。无论采用哪种生产系统，颗粒都通过遗传和/或化学工程进行官能化。在这里，我们回顾了各种方法的生产和功能化的病毒颗粒和VLP，我们讨论了每个系统的优点。
Viral particles and virus-like particles (VLPs) or capsids are becoming important vehicles and templates in bio-imaging, drug delivery and materials sciences. Viral particles are prepared by infecting the host organism but VLPs are obtained from cells that express a capsid protein. Some VLPs are disassembled and then re-assembled to incorporate a material of interest. Cell-free systems, which are amenable to manipulating the viral assembly process, are also available for producing viral particles. Regardless of the production system employed, the particles are functionalized by genetic and/or chemical engineering. Here, we review various methods for producing and functionalizing viral particles and VLPs, and we discuss the merits of each system.