Automated tests for measuring the effects of antidepressants in mice
Automated tests for measuring the effects of antidepressants in mice
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DOI：
10.1016/j.pbb.2004.03.014
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发表时间：
2004-06-01
影响因子：
3.6
通讯作者：
Lucki, I
中科院分区：
文献类型：
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作者：
Crowley, JJ;Jones, MD;Lucki, I
强迫游泳试验（FST）和悬尾试验（TST）被广泛用于测量抗抑郁药物的药理作用或小鼠应激诱发行为的变化。然而，不一致的评分技术和再现性差，可能会导致他们依赖于观察员的主观评分行为的变化。在本文中，自动化版本的小鼠FST和TST的特点和验证对观察员评级。对于FST，市售视频跟踪系统（SMART II; San Diego Instruments）测量小鼠以设定速度在充满水的圆筒中游泳的持续时间。对于TST，市售的自动化装置（Med Associates，St. Albans，VT）测量来自应变计的输入以检测悬挂在高架杆上的小鼠的运动。抗抑郁药地昔帕明对FST和TST不动性的剂量依赖性作用在CD-1小鼠中使用自动装置和录像带的手动评分来测量。使用两种方法获得了相似的剂量-反应曲线。然而，广泛的相关性评分员在FST表明，评分标准不同的个人评分员，尽管类似的指示。小鼠FST和TST的自动化版本现已推出，并提供了几个优势，包括有机会跨实验室标准化方法。(C)2004年爱思唯尔公司All rights reserved.
The forced swim test (FST) and the tail suspension test (TST) are used widely for measuring the pharmacological effects of antidepressant drugs or changes in stress-evoked behavior in mice. However, inconsistent scoring techniques and poor reproducibility may result from their reliance on subjective ratings by observers to score behavioral changes. In this paper, automated versions of the mouse FST and TST were characterized and validated against observer ratings. For the FST, a commercially available video tracking system (SMART II; San Diego Instruments) measured the duration that mice swam in water-filled cylinders at a set velocity. For the TST, a commercially available automated device (Med Associates, St. Albans, VT) measured input from a strain gauge to detect movements of mice suspended from an elevated bar. Dose-dependent effects of the antidepressant desipramine on FST and TST immobility were measured in CD-1 mice using both automated devices and manual scoring from videotapes. Similar dose-response curves were obtained using both methods. However, a wide range of correlations for raters in the FST indicated that scoring criteria varied for individual raters despite similar instructions. Automated versions of the mouse FST and TST are now available and provide several advantages, including an opportunity to standardize methods across laboratories. (C) 2004 Elsevier Inc. All rights reserved.