Cellular delivery of MRI contrast agents
Cellular delivery of MRI contrast agents
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DOI：
10.1016/j.chembiol.2004.03.003
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发表时间：
2004-03-01
影响因子：
--
通讯作者：
Meade, TJ
中科院分区：
文献类型：
--
作者：
Allen, MJ;MacRenaris, KW;Meade, TJ
磁共振成像（MRI）是一种强大的工具，用于获取不透明活体动物的图像，其优点是在同一标本上长时间跟踪事件。造影剂用于增强磁性相似但组织学不同的区域、组织和细胞。用于研究生物学问题的MRI造影剂的开发的主要障碍是穿过细胞膜递送药剂。在这里，我们描述了合成和体外测试的Gd（III）为基础的MRI造影剂含有不同长度的聚精氨酸低聚物能够渗透细胞膜。我们研究寡聚体的长度对T-1增强和细胞摄取的影响。此外，还探讨了孵育时间、浓度和细胞类型对摄取的影响。除了MRI体模研究外，还进行了毒性和洗脱研究。
Magnetic resonance imaging (MRI) is a powerful tool for acquiring images of opaque living animals with the benefit of tracking events over extended periods of time on the same specimen. Contrast agents are used to enhance regions, tissues, and cells that are magnetically similar but histologically distinct. A principal barrier to the development of MRI contrast agents for investigating biological questions is the delivery of agents across cellular membranes. Here, we describe the synthesis and in vitro testing of Gd(III)-based MRI contrast agents containing varying length polyarginine oligomers capable of permeating cell membranes. We examine the effect of the length of oligomer on T-1 enhancement and cellular uptake. Furthermore, the effect of incubation time, concentration, and cell type on uptake is explored. Toxicity and washout studies are performed in addition to MRI phantom studies.