In vitro fermentation of oat and barley derived β-glucans by human faecal microbiota
In vitro fermentation of oat and barley derived β-glucans by human faecal microbiota
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DOI：
10.1111/j.1574-6941.2008.00478.x
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发表时间：
2008-06-01
影响因子：
4.2
通讯作者：
Rastall, Robert A.
中科院分区：
文献类型：
--
作者：
Hughes, Simon A.;Shewry, Peter R.;Rastall, Robert A.
研究了人粪便微生物群对大麦[平均分子量（MM）为243、172和137 kDa]和燕麦（平均MM为230和150 kDa）中β-葡聚糖组分的发酵。对于每种底物，将级分补充至接种有来自三个供体的人粪便样品的pH控制厌氧分批培养发酵罐中，一式三份。通过荧光原位杂交监测微生物群的变化;列举的组为：双歧杆菌属、拟杆菌属和普雷沃氏菌属、溶组织梭菌亚组、瘤胃球菌-真杆菌-梭菌（REC）簇、乳杆菌-肠球菌属、变形菌簇和梭菌簇IX。短链脂肪酸和乳酸通过HPLC测定。梭溶组织菌亚群在所有血管中显著增加，梭菌群IX在所有组分中保持高种群。类杆菌-普雷沃氏菌组增加，但243 kDa的大麦和230 kDa的燕麦基板。一般而言，β-葡聚糖没有表现出明显的益生元潜力。SCFA分布（51：32：17;乙酸盐：丙酸盐：丁酸盐）被认为富含丙酸盐。在进一步的研究中，生产了聚合度为3-4的β-葡聚糖寡糖级分。这部分补充到小规模的粪便分批培养，并给出了显着增加的乳酸杆菌-肠球菌组，然而，这部分的益生元的潜力是边际相比，菊粉。
Fermentation of beta-glucan fractions from barley [average molecular mass (MM), of 243, 172, and 137 kDa] and oats (average MM of 230 and 150 kDa) by the human faecal microbiota was investigated. Fractions were supplemented to pH-controlled anaerobic batch culture fermenters inoculated with human faecal samples from three donors, in triplicate, for each substrate. Microbiota changes were monitored by fluorescent in situ hybridization; groups enumerated were: Bifidobacterium genus, Bacteroides and Prevotella group, Clostridium histolyticum subgroup, Ruminococcus-Eubacterium-Clostridium (REC) cluster, Lactobacillus-Enterococcus group, Atopobium cluster, and clostridial cluster IX. Short-chain fatty acids and lactic acid were measured by HPLC. The C. histolyticum subgroup increased significantly in all vessels and clostridial cluster IX maintained high populations with all fractions. The Bacteroides-Prevotella group increased with all but the 243-kDa barley and 230-kDa oat substrates. In general beta-glucans displayed no apparent prebiotic potential. The SCFA profile (51 : 32 : 17; acetate : propionate : butyrate) was considered propionate-rich. In a further study a beta-glucan oligosaccharide fraction was produced with a degree of polymerization of 3-4. This fraction was supplemented to small-scale faecal batch cultures and gave significant increases in the Lactobacillus-Enterococcus group; however, the prebiotic potential of this fraction was marginal compared with that of inulin.