An integrated microfluidic platform for selective and real-time detection of thrombin biomarkers using a graphene FET
An integrated microfluidic platform for selective and real-time detection of thrombin biomarkers using a graphene FET
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DOI：
10.1039/d0an00251h
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发表时间：
2020-07-07
期刊：
影响因子：
4.2
通讯作者：
Song, Edward
中科院分区：
文献类型：
--
作者：
Khan, Niazul, I;Mousazadehkasin, Mohammad;Song, Edward
芯片实验室技术提供了一个理想的平台，用于低成本，可靠和易于使用的诊断疾病和其他健康问题的早期筛查所需的关键生物标志物。在这项工作中，石墨烯场效应晶体管（GFET）功能化的目标结合适体被用作生物传感器检测凝血酶蛋白生物标志物。此外，该GFET与微流体装置集成，用于在检测限、灵敏度和连续监测方面增强传感性能。在此平台下，实现了皮摩尔检测限用于测量凝血酶;在我们的实验中测量低至2.6 pM。进行FTIR、拉曼和UV-Vis光谱测量以确认器件功能化步骤。根据浓度依赖性校准曲线，获得K（D）= 375.8 pM的解离常数。还在恒定栅极电压（V-GS）下进行连续实时测量，以观察当分析物被引入装置时传感器的瞬态响应。通过用各种浓度的溶菌酶蛋白质挑战GFET生物传感器来评估和确认传感器平台的靶选择性。结果表明，该器械技术有可能用作测量床旁应用的临床相关生物标志物的通用诊断平台。
Lab-on-a-chip technology offers an ideal platform for low-cost, reliable, and easy-to-use diagnostics of key biomarkers needed for early screening of diseases and other health concerns. In this work, a graphene field-effect transistor (GFET) functionalized with target-binding aptamers is used as a biosensor for the detection of thrombin protein biomarker. Furthermore, this GFET is integrated with a microfluidic device for enhanced sensing performances in terms of detection limit, sensitivity, and continuous monitoring. Under this platform, a picomolar limit of detection was achieved for measuring thrombin; in our experiment measured as low as 2.6 pM. FTIR, Raman and UV-Vis spectroscopy measurements were performed to confirm the device functionalization steps. Based on the concentration-dependent calibration curve, a dissociation constant ofK(D)= 375.8 pM was obtained. Continuous real-time measurements were also conducted under a constant gate voltage (V-GS) to observe the transient response of the sensor when analyte was introduced to the device. The target selectivity of the sensor platform was evaluated and confirmed by challenging the GFET biosensor with various concentrations of lysozyme protein. The results suggest that this device technology has the potential to be used as a general diagnostic platform for measuring clinically relevant biomarkers for point-of-care applications.