Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
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DOI：
10.1126/science.1190719
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发表时间：
2010-07-02
期刊：
影响因子：
56.9
通讯作者：
Venter, J. Craig
中科院分区：
文献类型：
--
作者：
Gibson, Daniel G.;Glass, John I.;Venter, J. Craig
我们报告了从数字化基因组序列信息开始的1.08兆碱基对丝状支原体JCVI-syn 1.0基因组的设计、合成和组装，并将其移植到M.山羊受体细胞产生新的M.只由合成染色体控制的蕈状细胞。细胞中唯一的DNA是设计的合成DNA序列，包括“水印”序列和其他设计的基因缺失和多态性，以及在构建过程中获得的突变。新细胞具有预期的表型特性，并能够连续自我复制。
We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.