EFFECT OF DEUTERIUM SUBSTITUTION IN SYMPATHOMIMETIC AMINES ON ADRENERGIC RESPONSES
EFFECT OF DEUTERIUM SUBSTITUTION IN SYMPATHOMIMETIC AMINES ON ADRENERGIC RESPONSES
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DOI：
10.1126/science.133.3446.102
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发表时间：
1961-01-01
期刊：
影响因子：
56.9
通讯作者：
BURBA, J
中科院分区：
文献类型：
--
作者：
BELLEAU, B;BURBA, J
研究发现，用氘取代酪胺和色胺的α-氢，可显著增强这些胺通常产生的血压效应和瞬膜收缩。解释的基础上的动力学同位素效应的单胺氧化酶的水平上的结果，并清楚地建立这种酶的重要性，在限制反应时，酪胺和色胺参与。用[α]，[α]-双氘酪胺（[α]，[α]-D2-酪胺）观察到的氘同位素效应仅用单氘酪胺的一种光学异构体再现。这表明该酶具有高度的光学特异性。使用l-双氘代-去甲肾上腺素表明去甲肾上腺素不能在效应细胞水平上被酶攻击。
It was discovered that replacement of the [alpha]-hydrogens of tyramine and tryptamine by deuterium produces a marked intensification of the blood pressure effects and nictitating membrane contraction normally produced by these amines. The results are interpreted on the basis of kinetic isotope effects at the level of monoamine oxidase and clearly establish the importance of this enzyme in the limitation of responses when tyramine and tryptamine are involved. The observed deuterium isotope effects with [alpha],[alpha]-bis-deuterotyramine ([alpha], [alpha]-D2-tyramine) have been reproduced with only one of the optical isomers of monodeuterotyramine. This establishes that the enzyme displays a high degree of optical specificity. The use of l-bisdeutero-norepinephrine revealed that norepinephrine cannot be attacked by the enzyme at the effector cell level.