Expression profiling following traumatic brain injury: A review
Expression profiling following traumatic brain injury: A review
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DOI：
10.1023/a:1020973308941
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发表时间：
2002-10-01
影响因子：
4.4
通讯作者：
McIntosh, TK
中科院分区：
文献类型：
--
作者：
Marciano, PG;Eberwine, JH;McIntosh, TK
创伤性脑损伤（TBI）激发了一系列复杂的假定的自我破坏和神经保护细胞级联反应。据推测，受损大脑中细胞的基因组反应是这些级联反应的基础。用于分析脑创伤后差异基因表达的传统方法表明，即刻早期基因、细胞因子、转录因子和神经营养因子都可以参与脑对损伤的主动和定向反应，并且可以同时进行。正是这种相互关联的分子机制的复杂性和多样性，需要新的方法来全面和平行评估推定的以及新的基因靶点。DNA微阵列技术的最新进展使得能够同时评估数千个基因和随后产生的大量与CNS损伤相关的生物学数据。这项新兴技术可以进一步了解目前公认的分子级联反应，并确定整个创伤后时期发生的新分子机制。阐明TBI后病理后遗症的基因表达的复杂变化在未来治疗药物的设计中具有核心重要性。
Traumatic brain injury (TBI) elicits a complex sequence of putative autodestructive and neuroprotective cellular cascades. It is hypothesized that the genomic responses of cells in the injured brain serve as the basis for these cascades. Traditional methods for analyzing differential gene expression following brain trauma demonstrate that immediate early genes, cytokines, transcription factors, and neurotrophic factors can all participate in the brain's active and directed response to injury, and may do so concurrently. It is this complexity and multiplicity of interrelated molecular mechanisms that has demanded new methods for comprehensive and parallel evaluation of putative as well as novel gene targets. Recent advances in DNA microarray technology have enabled the simultaneous evaluation of thousands of genes and the subsequent generation of massive amounts of biological data relevant to CNS injury. This emerging technology can serve to further current knowledge regarding recognized molecular cascades as well as to identify novel molecular mechanisms that occur throughout the post-traumatic period. The elucidation of the complex alterations in gene expression underlying the pathological sequelae following TBI is of central importance in the design of future therapeutic agents.