Osteocyte Characterization on Polydimethylsiloxane Substrates for Microsystems Applications.
Osteocyte Characterization on Polydimethylsiloxane Substrates for Microsystems Applications.
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DOI：
10.4028/www.scientific.net/jbbte.16.27
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Saunders MM
中科院分区：
文献类型：
--
作者：
York SL;Arida AR;Shah KS;Sethu P;Saunders MM
在体内，骨细胞位于骨陷窝中，即矿化骨内的透镜状空腔中。这些细胞通过称为间隙连接的物理通道相互连接，并与表面居住的成骨细胞连接。已经提出骨细胞感知施加于骨的机械负荷并将该信号传递给破骨细胞和成骨细胞。目前在体外和体内模型的力学转导面临时间和空间的障碍。基于聚二甲基硅氧烷（PDMS）的微制造技术的最新进展可能能够克服这些障碍中的一些。然而，在骨骼研究领域可以有效地利用微系统技术，必须完成基础的基础工作。这项研究的特点是骨细胞的行为与胶原蛋白I型（CTI）涂覆的PDMS和骨细胞力学转导研究提供了框架，使用微系统。目的是通过将骨细胞的行为与标准玻璃基质进行比较，确定骨细胞是否受到基质材料的不利影响。此外，最佳的培养条件和时间点生长的骨细胞的PDMS基板上确定。这项研究的结果表明，使用PDMS不会对骨细胞的行为产生不利影响。此外，结果表明，骨细胞应在实验前培养不少于72小时，以建立和维持表型特征。这些结果完成了必要的基础，为进一步研究骨细胞在微系统建模利用PDMS。
In the body, osteocytes reside in lacunae, lenticular shaped cavities within mineralized bone. These cells are linked to each other and surface-residing osteoblasts via physical channels known as gap junctions. It has been suggested that osteocytes sense mechanical load applied to bone and relay that signal to osteoclasts and osteoblasts. Current in vitro and in vivo models of mechanotransduction face temporal and spatial barriers. Recent advances in polydimethylsiloxane (PDMS) based microfabrication techniques may be able to overcome some of these hurdles. However, before the bone research field can effectively utilize microsystems techniques, fundamental groundwork must be completed. This study characterized the behaviour of osteocytes on PDMS coated with collagen type I (CTI) and provides the framework for bone cell mechanotransduction studies using microsystems. The goal was to determine whether osteocytes were adversely affected by the substrate material by comparing their behaviour to a standard glass substrate. In addition, optimal culture conditions and time points for growing osteocytes on PDMS substrates were determined. Results of this study suggested that use of PDMS does not adversely affect osteocyte behaviour. Furthermore, the results demonstrated that osteocytes should be cultured for no less than 72 hours prior to experimentation to allow the establishment and maintenance of phenotypic characteristics. These results completed essential groundwork necessary for further studies regarding osteocytes in microsystems modelling utilizing PDMS.