Two members of the Arabidopsis CLC (chloride channel) family, AtCLCe and AtCLCf, are associated with thylakoid and Golgi membranes, respectively
Two members of the Arabidopsis CLC (chloride channel) family, AtCLCe and AtCLCf, are associated with thylakoid and Golgi membranes, respectively
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DOI：
10.1093/jxb/erm187
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发表时间：
2007-10-01
影响因子：
6.9
通讯作者：
Ephritikhine, Genevieve
中科院分区：
文献类型：
--
作者：
Marmagne, Anne;Vinauger-Douard, Marion;Ephritikhine, Genevieve
尽管大量的证据表明阴离子通道在植物中起着主要的生理作用，但到目前为止发现的基因数量很少，限制了人们对其生物学功能的了解。在拟南芥基因组中可以鉴定出7个氯离子通道(CLC)成员，其中AtCLCe和AtCLCf与细菌CLC的亲缘关系比其他植物的CLC更近。结果表明，AtCLCe是针对叶绿体中的类囊体膜的，并且与这种亚细胞定位一致，clce突变体表现出与光合作用活性相关的表型。AtCLCf蛋白定位于高尔基体膜上，并在功能上补充酵母gef1突变体，该突变株在编码高尔基体相关蛋白的单个ClC基因中被破坏。
Though numerous pieces of evidence point to major physiological roles for anion channels in plants, progress in the understanding of their biological functions is limited by the small number of genes identified so far. Seven chloride channel (CLC) members could be identified in the Arabidopsis genome, amongst which AtCLCe and AtCLCf are both more closely related to bacterial CLCs than the other plant CLCs. It is shown here that AtCLCe is targeted to the thylakoid membranes in chloroplasts and, in agreement with this subcellular localization, that the clce mutants display a phenotype related to photosynthesis activity. The AtCLCf protein is localized in Golgi membranes and functionally complements the yeast gef1 mutant disrupted in the single CLC gene encoding a Golgi-associated protein.