Stiffness measurement of nanosized liposomes using solid-state nanopore sensor with automated recapturing platform
Stiffness measurement of nanosized liposomes using solid-state nanopore sensor with automated recapturing platform
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DOI：
10.1002/elps.201800476
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发表时间：
2019-05-01
期刊：
影响因子：
2.9
通讯作者：
Kim, Min Jun
中科院分区：
文献类型：
--
作者：
Lee, Jung Soo;Saharia, Jugal;Kim, Min Jun
本文描述了一种方法来衡量纳米脂质体的刚度-纳米囊泡-使用定制的再捕获平台耦合到固态纳米孔传感器。重新捕获平台通过在易位实例之后立即自动反转电压极性以使相同的分析物重新易位通过纳米孔来多次电描绘给定的脂质体囊泡-与常规的纳米孔平台相比，通过多次分析相同的颗粒在分子水平上提供更好的统计洞察。捕获频率取决于具有较低电压的施加电压（即，在更高的电压（> 100 mV）下，允许比在更高的电压（> 200 mV）下更高的重新捕获实例，因为颗粒离开纳米孔捕获半径的概率随着电压而增加。通过将两个电压极性下的归一化相对电流阻断（I/I 0）与刚性颗粒的归一化相对电流阻断（I/I 0）进行比较来推断形状变形，即，聚苯乙烯珠。我们发现，脂质体变形，采用一个扁长的形状在较高的电压。该平台可进一步应用于其他类型软物质的刚度研究，病毒、外泌体、内体，并通过控制药物囊泡的刚度来加速药物学中用于增加药物包装和解包机制的潜在研究。
This paper describes a method to gauge the stiffness of nanosized liposomes - a nanoscale vesicle - using a custom-made recapture platform coupled to a solid-state nanopore sensor. The recapture platform electrically profiles a given liposome vesicle multiple times through automated reversal of the voltage polarity immediately following a translocation instance to re-translocate the same analyte through the nanopore - provides better statistical insight at the molecular level by analyzing the same particle multiple times compared to conventional nanopore platforms. The capture frequency depends on the applied voltage with lower voltages (i.e., 100mV) permitting higher recapture instances than at higher voltages (>200mV) since the probability of particles exiting the nanopore capture radius increases with voltage. The shape deformation was inferred by comparing the normalized relative current blockade (I/I0) at the two voltage polarities to that of a rigid particle, i.e., polystyrene beads. We found that liposomes deform to adopt a prolate shape at higher voltages. This platform can be further applied to investigate the stiffness of other types of soft matters, e.g., virus, exosomes, endosomes, and accelerate the potential studies in pharmaceutics for increasing the drug packing and unpacking mechanism by controlling the stiffness of the drug vesicles.