Effect of group vs. single housing on phenotypic variance in C57BL/6J mice
Effect of group vs. single housing on phenotypic variance in C57BL/6J mice
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DOI：
10.1038/oby.2002.57
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发表时间：
2002-05-01
期刊：
影响因子：
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通讯作者：
Desmond, R
中科院分区：
文献类型：
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作者：
Nagy, TR;Krzywanski, D;Desmond, R
目的：确定群体饲养是否影响高度近交小鼠品系的身体成分参数的变化。研究方法和程序：从Jackson实验室获得30只3周龄的雄性C57BL/6J小鼠。将 15 只小鼠单独圈养，将 15 只小鼠按每笼 5 只为一组圈养。动物随意饲喂并在22℃、12:12小时光照/黑暗光周期的同一房间中饲养9周。处死动物，通过 DXA 测定脂肪量、软瘦组织量、骨矿物质密度 (BMD) 和骨矿物质含量 (BMC)。尸检时，获得配对附睾脂肪垫、配对腹膜后脂肪垫、右腹股沟脂肪垫、肝脏、肾脏、配对睾丸和精囊的重量。 结果：相对于单独饲养的小鼠，群养的小鼠在体脂百分比、睾丸重量和 BMC 方面表现出显着更大的差异。群养小鼠的肝脏重量和骨密度往往表现出更大的差异。与群养小鼠相比，单独饲养的小鼠体型较小，软组织质量和 BMC 较少，BMD 也较低。讨论：这些结果表明，就身体成分参数而言，单独饲养的小鼠比群养的小鼠更相似，很可能是因为环境（主要是行为/社会）影响减少。因此，单独饲养的小鼠可能比群养的小鼠更能代表基因型对身体成分的影响。其他近交系小鼠是否对饲养条件表现出类似的反应尚不清楚。
Objective: To determine if group housing affects the variance of body composition parameters in a highly inbred mouse strain.Research Methods and Procedures: Thirty 3-week-old male C57BL/6J mice were obtained from the Jackson Laboratory. Fifteen mice were housed individually, and 15 mice were housed in groups of 5/cage. Animals were fed ad libitum and maintained in the same room under a 12:12-hour light/dark photoperiod at 22 degreesC for 9 weeks. Animals were killed, and fat mass, soft-lean tissue mass, bone mineral density (BMD), and bone mineral content (BMC) were determined by DXA. At necropsy, weights of the paired epididymal fat pads, paired retroperitoneal fat pads, right inguinal fat pad, liver, kidneys, paired testes, and seminal vesicles were obtained.Results: Relative to mice housed singly, group-housed mice showed significantly greater variance in percentage of body fat, testes weight, and BMC. Group-housed mice tended to show greater variance in liver weights and BMD. Mice housed singly were smaller, had less soft-lean tissue mass and BMC, and lower BMD when compared with group-housed mice.Discussion: These results suggest that with respect to body composition parameters, mice housed singly are more similar to one another than are group-housed mice, most likely because of a reduction in environmental (predominately behavioral/social) effects. Thus, mice housed singly may be more representative of genotypic effects on body composition than group-housed mice. Whether other inbred strains of mice show similar responses to housing condition is unknown.