Phenotypic and genetic relationships of bovine natural antibodies binding keyhole limpet hemocyanin in plasma and milk
Phenotypic and genetic relationships of bovine natural antibodies binding keyhole limpet hemocyanin in plasma and milk
复制标题
DOI：
10.3168/jds.2014-8818
复制
发表时间：
2015-04-01
影响因子：
3.5
通讯作者：
van der Poel, J. J.
中科院分区：
文献类型：
--
作者：
de Klerk, B.;Ducro, B. J.;van der Poel, J. J.
为了改善奶牛的健康状况（恢复力），天然抗体（NAb）的水平可能是有用的。本研究的目的是比较牛奶和血浆样本中NAb的水平，并估计遗传参数。使用ELISA测定了2，919头奶牛血浆和乳汁中NAb IgM和IgG同种型结合钥孔血蓝蛋白的滴度。分析表明，NAb水平显着增加，在牛奶中的奇偶校验，而他们保持不变的血浆。牛奶和血浆中NAb水平之间存在中度正表型相关性：IgG为0.18，IgM为0.40。这表明，牛奶和血浆中的NAb可能反映奶牛健康状况的不同方面。然而，高的遗传相关性被发现在牛奶和血浆中的NAb：0.81的IgG和0.79的IgM。血浆中测得的NAb的遗传力（括号中的SE）[IgG为0.15（0.05），IgM为0.25（0.06）]高于乳汁中测得的NAb的遗传力[IgG为0.08（0.03），IgM为0.23（0.05）]。我们的研究结果表明，在牛奶和血浆中测得的NAb是可遗传的，可能有一个共同的遗传背景，这表明在牛奶中测得的NAb水平可能是有用的抗病性的遗传改良。
To improve the health status (resilience) of dairy cows, levels of natural antibodies (NAb) might be useful. The objective of the present study was to compare levels and to estimate genetic parameters for NAb measured in milk and plasma samples. Titers of NAb IgM and IgG isotype-binding keyhole limpet hemocyanin of 2,919 cows, in both plasma and milk, were measured using ELISA. Analysis revealed that NAb levels in milk significantly increased with parity, whereas they remained constant in plasma. Moderate positive phenotypic correlations were found between NAb levels in milk and in plasma: 0.18 for IgG and 0.40 for IgM. This indicates that NAb from milk and plasma might reflect different aspects of dairy cow health status. However, high genetic correlations were found for NAb in milk and plasma: 0.81 for IgG and 0.79 for IgM. Heritabilities (SE in parentheses) for NAb measured in plasma [0.15 (0.05) for IgG and 0.25 (0.06) for IgM] were higher than heritabilities of NAb measured in milk [0.08 (0.03) for IgG and 0.23 (0.05) for IgM]. Our results indicate that NAb measured in milk and plasma are heritable and likely have a common genetic background, suggesting that NAb levels measured in milk might be useful for genetic improvement of disease resistance.