Involvement of polyubiquitin chains via specific chain linkages in stress response in mammalian cells
Involvement of polyubiquitin chains via specific chain linkages in stress response in mammalian cells
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DOI：
10.1248/bpb.28.2315
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发表时间：
2005-12-01
影响因子：
2
通讯作者：
Yokosawa, H
中科院分区：
文献类型：
--
作者：
Fujimuro, M;Nishiya, T;Yokosawa, H
多聚泛素化在各种蛋白酶体依赖性和非依赖性细胞事件中起着关键作用。为了阐明哺乳动物细胞中通过特定链连接形成的多聚泛素链在应激反应中的作用，我们建立了N1H3T3稳定细胞系，这些细胞系能够有条件地表达K29R、K48R和K63R泛素突变体，其中泛素的Lys29、Lys48和Lys63残基已被改变为Arg，并且我们检查了各种应激对其细胞活力的影响。K63R泛素突变体的表达降低了乙醇、H2O2和甲基磺酸甲酯（MMS）暴露后的细胞活力，而K48R突变体的表达降低了热休克以及乙醇、H2O2和MMS暴露后的细胞活力。因此，这些结果表明，通过特定的链连接形成的多聚泛素链参与哺乳动物细胞中的相应应激反应。
Polyubiquitination plays key roles in various proteasome-dependent and independent cellular events. To elucidate roles in stress response of polyubiquitin chains formed via specific chain linkages in mammalian cells, we established N1H3T3 stable cell lines that are capable of conditionally expressing K29R, K48R and K63R ubiquitin mutants, in which the Lys29, Lys48 and Lys63 residues of ubiquitin had been changed to Arg, and we examined the effects of various stresses on their cell viabilities. The expression of K63R ubiquitin mutant decreased viability of the cells post-exposed to ethanol, H2O2 and methyl methanesulfonate (MMS), while that of K48R mutant decreased viability of the cells post-exposed to heat shock as well as ethanol, H2O2 and MMS. Thus, these results suggest that polyubiquitin chains formed via specific chain linkages are involved in the respective stress responses in mammalian cells.