Cytomegalovirus (CMV) IE1-and pp65-specific CD8+ T cell responses broaden over time after primary CMV infection in infants
Cytomegalovirus (CMV) IE1-and pp65-specific CD8+ T cell responses broaden over time after primary CMV infection in infants
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DOI：
10.1086/518042
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发表时间：
2007-06-15
影响因子：
6.4
通讯作者：
Luzuriaga, Katherine
中科院分区：
文献类型：
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作者：
Gibson, Laura;Dooley, Sheryl;Luzuriaga, Katherine
巨细胞病毒（CMV）感染仍然是幼儿发病和死亡的重要原因。我们之前已经证明，在先天性或出生后感染 CMV 的儿童中，很容易检测到 CD8+ T 细胞对 CMV pp65 或 IE1 蛋白的反应。在这里，我们在研究开始时进一步表征了 7 名 < 6 个月大的婴儿中这些反应的肽特异性的演变。 13 个 pp65 和 15 个 IE1 肽（中位数，5 个肽/婴儿）成为目标，大多数 (61%) 代表以前未报道过的序列。尽管 CMV 病毒血症已被清除，但随着时间的推移，肽特异性仍保持稳定或扩大。没有观察到肽识别的丧失。具有最高功能性肽亲和力的反应不一定最早被检测到。这些数据提供了额外的证据，表明年幼的婴儿可以产生不同的 CMV 特异性 CD8(+) T 细胞反应，但表明早期反应可能表现出相对集中的肽特异性和较低的肽亲合力。
Cytomegalovirus (CMV) infection remains a significant cause of morbidity and mortality in young children. We have previously shown that CD8(+) T cell responses to CMV pp65 or IE1 protein were readily detectable in children with congenital or postnatal CMV infection. Here, we have further characterized the evolution of the peptide specificity of these responses in 7 infants < 6 months of age at the start of the study. Thirteen pp65 and 15 IE1 peptides (median, 5 peptides/infant) were targeted, and most (61%) represented sequences not previously reported. Peptide specificity remained stable or broadened over time despite the clearance of CMV viremia. Loss of peptide recognition was not observed. Responses with the highest functional peptide avidity were not necessarily detected earliest. These data provide additional evidence that young infants can generate diverse CMV-specific CD8(+) T cell responses but show that early responses may exhibit relatively focused peptide specificity and lower peptide avidity.