The Spatiotemporal Limits of Developmental Erk Signaling.
The Spatiotemporal Limits of Developmental Erk Signaling.
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DOI：
10.1016/j.devcel.2016.12.002
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发表时间：
2017-01-23
影响因子：
11.8
通讯作者：
Toettcher JE
中科院分区：
文献类型：
--
作者：
Johnson HE;Goyal Y;Pannucci NL;Schüpbach T;Shvartsman SY;Toettcher JE
动物发育的特点是在胚胎内精确的位置和时间发生信号事件，然而确定何时何地需要这种精确的胚胎发生一直是一个长期的挑战。在这里，我们针对Erk信号这个关键的发育模式线索来解决这个问题。我们描述了一种在体内具有高时空精度激活Erk的光遗传系统。在果蝇中实施该系统，我们发现胚胎发生对异位Erk信号非常稳健，除了受精后1至4小时，此时干扰Erk通路激活的空间范围会导致模式和形态发生的严重破坏。在发育后期，异位信号的影响被缓冲，至少部分是通过组合机制。我们的方法可以用于系统地探索Erk通路和并发信号的差异贡献，从而对发育信号有更定量的了解。
Animal development is characterized by signaling events that occur at precise locations and times within the embryo, yet determining when and where such precision is needed for proper embryogenesis has been a longstanding challenge. Here we address this question for Erk signaling, a key developmental patterning cue. We describe an optogenetic system for activating Erk with high spatiotemporal precision in vivo. Implementing this system in Drosophila, we find that embryogenesis is remarkably robust to ectopic Erk signaling, except from 1 to 4 hours post fertilization when perturbing the spatial extent of Erk pathway activation leads to dramatic disruptions of patterning and morphogenesis. Later in development, the effects of ectopic signaling are buffered, at least in part by combinatorial mechanisms. Our approach can be used to systematically probe the differential contributions of the Erk pathway and concurrent signals, leading to a more quantitative understanding of developmental signaling.