IMMUNOLOGICAL UNRESPONSIVENESS INDUCED BY CRYPTOCOCCAL CAPSULAR POLYSACCHARIDE ASSAYED BY HEMOLYTIC PLAQUE TECHNIQUE
IMMUNOLOGICAL UNRESPONSIVENESS INDUCED BY CRYPTOCOCCAL CAPSULAR POLYSACCHARIDE ASSAYED BY HEMOLYTIC PLAQUE TECHNIQUE
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DOI：
10.1128/iai.5.6.896-901.1972
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发表时间：
1972-01-01
影响因子：
3.1
通讯作者：
COZAD, GC
中科院分区：
文献类型：
--
作者：
MURPHY, JW;COZAD, GC
大量研究表明隐球菌荚膜多糖可引起免疫瘫痪。为了研究这种可能性，小鼠给予不同浓度的纯化隐球菌多糖，然后在14天后用弗氏不完全佐剂中的相同材料进行攻击免疫。抗隐球菌凝集素滴度在多糖治疗后和攻击免疫后的不同时期进行了测定。同时用溶血空斑法测定脾细胞产生抗隐球菌多糖抗体的数量。数据表明，在耐受诱导之前存在暂时的免疫应答。在给予最大剂量多糖的动物中，“体内”中和是导致短暂反应期间血清凝集素滴度低的原因。认为荚膜多糖在最高浓度下诱导免疫无反应性，因为攻击免疫没有刺激空斑形成细胞（PFC）数量的增加。在最初注射最低浓度多糖的动物中发现PFC数量增加了六倍。这些结果支持耐受性是由于终末分化而没有免疫活性细胞增殖的观点。在本实验条件下，瘫痪小鼠中明显的免疫机制中枢失效是暂时的。
Numerous studies have suggested that cryptococcal capsular polysaccharide could induce an immunological paralysis. To investigate this possibility, mice were given various concentrations of purified cryptococcal polysaccharide and then 14 days later were challenge-immunized with the same material in Freund's incomplete adjuvant. Anticryptococcal agglutinin titers were determined at various periods after polysaccharide treatment and after challenge immunization. At the same periods the hemolytic plaque technique was used to determine the number of spleen cells capable of producing antibody against cryptococcal polysaccharide. The data indicated that there was a transitory immune response which preceded tolerance induction. In animals given the largest doses of polysaccharide, “in vivo” neutralization was responsible for low serum agglutinin titers during the transitory response. The capsular polysaccharide was considered to have induced immunological unresponsiveness at the highest concentration, because challenge immunization did not stimulate an increase in the number of plaque-forming cells (PFC). A sixfold increase in numbers of PFC was found in animals injected initially with the lowest concentration of polysaccharide. These results support the idea that tolerance was due to terminal differentiation without proliferation of the immunocompetent cells. The central failure of the immune mechanism which was apparent in the paralyzed mice was temporary under the conditions of this experiment.