Development of a reverse genetics system for snakehead vesiculovirus (SHVV)
Development of a reverse genetics system for snakehead vesiculovirus (SHVV)
复制标题
蛇头水泡病毒（SHVV）反向遗传学系统的开发
DOI：
10.1016/j.virol.2018.10.002
复制
发表时间：
2019-01-02
期刊：
影响因子：
3.7
通讯作者：
Tu, Jiagang
中科院分区：
文献类型：
--
作者：
Feng, Shuangshuang;Su, Jianguo;Tu, Jiagang
蛇头囊状病毒（SHVV）是一种从患病的杂交黑头鱼（斑母黑头鱼和雄黑头鱼）中分离到的新型横纹病病毒，在中国黑头鱼养殖中造成了严重的经济损失。为了更好地了解SHVV的致病性，我们利用人和鱼细胞建立了SHVV的反向遗传系统。将编码SHVV全长抗基因组RNA或支持蛋白(核蛋白(N)、磷酸化蛋白（P)、大聚合酶(L)）的4个质粒共转染人293T细胞，然后在Channel catfish ovary （CCO）细胞中培养。我们还获得了一个表达增强型绿色荧光蛋白（EGFP）的重组SHVV，将其插入到SHVV糖蛋白(G)基因的3'非编码区（NCR）。我们的研究为揭示SHVV的致病性提供了一个潜在的工具，并为利用人293T和鱼细胞拯救其他鱼类病毒提供了一个模板。
Snakehead vesiculovirus (SHVV) is a new rhabdovirus isolated from diseased hybrid snakehead fish (Channa maculate female x Channa argus (male) and has caused serious economic losses in snakehead fish culture in China. To better understand the pathogenicity of SHVV, we developed a reverse genetics system for SHVV by using human and fish cells. In detail, human 293T cells were co-transfected with four plasmids encoding the fulllength SHVV antigenomic RNA or the supporting proteins including nucleoprotein (N), phosphoprotein (P), and large polymerase (L), followed by the cultivation in Channel catfish ovary (CCO) cells. We also rescued a recombinant SHVV expressing enhanced green fluorescent protein (EGFP), which was inserted into the 3' non-coding region (NCR) of the glycoprotein (G) gene of SHVV. Our study provides a potential tool for unveiling the pathogenicity of SHVV and a template for the rescue of other fish viruses by using both human 293T and fish cells.