Gene expression profile analysis indicate SEPALLATA3 and AGL15 potentially involved in arabidopsis silique dehiscence by regulating glycosyl hydrolase
Gene expression profile analysis indicate SEPALLATA3 and AGL15 potentially involved in arabidopsis silique dehiscence by regulating glycosyl hydrolase
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基因表达谱分析表明 SEPALLATA3 和 AGL15 可能通过调节糖基水解酶参与拟南芥开裂
DOI：
10.1007/s12374-016-0567-5
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发表时间：
2016-04-01
影响因子：
2.9
通讯作者：
Wu, Hong
中科院分区：
文献类型：
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作者：
Jiang, Xingshan;He, Hanjun;Wu, Hong
果实开裂是某些经济作物的重要发育过程，其显著影响作物产量，并且涉及数千个基因和相应生物学过程的调节改变。果实开裂的调控是复杂的，虽然一些调控基因已被表征，但总体机制仍然未知。在这项研究中，我们使用微阵列分析筛选基因的表达模式在拟南芥角果开裂的三个阶段（阶段11，15和17）。检测差异表达基因（DEG），结合功能富集分析和短时间序列表达挖掘（STEM）聚类分析，探讨果实开裂过程中基因表达的整体变化及其相关功能。对这些基因簇的转录因子富集分析表明，SEPALLATA 3和AGL 15这两个转录因子可能是角果开裂的关键调控因子，因为它们靶向与细胞壁降解相关的糖基水解酶。两者合计，我们的数据使连接之间绘制specic生物功能，基因和TF，这支持网络模型的发展，以阐明角果开裂的过程。
Fruit dehiscence is an essential developmental process for certain economic crops that dramatically impacts crop yields and involves the altered regulation of thousands of genes and corresponding biological processes. The regulation of fruit dehiscence is complex; although some regulatory genes have been characterized, the overall mechanism is still unknown. In this study, we used microarray analysis to screen the expression patterns of genes during three stages of Arabidopsis thaliana silique dehiscence (stages 11, 15, and 17). Differentially expressed genes (DEGs) were detected, and the combination of functional enrichment analysis with Short Time-series Expression Miner (STEM) clustering analysis was used to explore the overall gene expression changes and related functions during fruit dehiscence. A total of 9 specific gene expression clusters and corresponding functions were found. Moreover, transcription factor (TF) enrichment analysis for these gene clusters showed that two TFs (SEPALLATA3 and AGL15) maybe key regulatory factors for silique dehiscence because they target many glycosyl hydrolases in a certain cluster related to cell wall degradation. Taken together, our data enable connections to be drawn among specic biological functions, genes and TFs, which supports the development of network models to elucidate the process of silique dehiscence.