The impact of dehydration rate on the production and cellular location of reactive oxygen species in an aquatic moss
The impact of dehydration rate on the production and cellular location of reactive oxygen species in an aquatic moss
复制标题
DOI：
10.1093/aob/mcs180
复制
发表时间：
2012-10-01
期刊：
影响因子：
4.2
通讯作者：
Barreno, Eva
中科院分区：
文献类型：
--
作者：
de Carvalho, Ricardo Cruz;Catala, Myriam;Barreno, Eva
水生苔藓退热需要缓慢的脱水速度才能在干燥事件中幸存下来。利用激光共聚焦显微镜和ROS特异化学探针2，7-二氯二氢荧光素二乙酸酯对水生苔藓细胞内ROS的产生进行了研究。过氧化氢的产生也是定量的，并对其细胞位置进行了评估。缓慢干燥的细胞的复水与较低的ROS产生有关，从而减少了细胞的损伤量，提高了细胞的存活率。高耗氧爆发伴随着复水的初期阶段，可能是由于ROS产生的暴发。缓慢的脱水速率可能会诱导细胞保护机制，从而限制ROS的产生并减少氧化暴发，从而减少复水后受损和死亡的细胞数量。
The aquatic moss Fontinalis antipyretica requires a slow rate of dehydration to survive a desiccation event. The present work examined whether differences in the dehydration rate resulted in corresponding differences in the production of reactive oxygen species (ROS) and therefore in the amount of cell damage.Intracellular ROS production by the aquatic moss was assessed with confocal laser microscopy and the ROS-specific chemical probe 2,7-dichlorodihydrofluorescein diacetate. The production of hydrogen peroxide was also quantified and its cellular location was assessed.The rehydration of slowly dried cells was associated with lower ROS production, thereby reducing the amount of cellular damage and increasing cell survival. A high oxygen consumption burst accompanied the initial stages of rehydration, perhaps due to the burst of ROS production.A slow dehydration rate may induce cell protection mechanisms that serve to limit ROS production and reduce the oxidative burst, decreasing the number of damaged and dead cells due upon rehydration.