Real-time virus trapping and fluorescent imaging in microfluidic devices
Real-time virus trapping and fluorescent imaging in microfluidic devices
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DOI：
10.1021/nl034987p
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发表时间：
2004-02-01
期刊：
影响因子：
10.8
通讯作者：
Bashir, R
中科院分区：
文献类型：
--
作者：
Akin, D;Li, HB;Bashir, R
我们已经在微流控生物芯片中使用介电泳过滤器来从流中捕获病毒颗粒并实时成像。通过使用双重或三重标记荧光标记颗粒来进行捕获的验证。这些非机械过滤器在样品制备、纯化和从混合样品中浓缩病毒颗粒方面非常有价值。所描述的成像方法可用于纳米级病毒颗粒的实时成像，以分析这些颗粒在微米和纳米级传感器内的捕获、检测和表征。
We have used dielectrophoretic filters within microfluidic biochips to capture from a flow and image virus particles in real-time. The verification of capture was performed by fluorescently labeling the particles using dual or triple labeling. These nonmechanical filters can be very valuable in the sample preparation, purification, and concentration of viral particles from a mixed sample. The described imaging methodology can be used for real-time imaging of nanometer scale virus particles for analysis of capture, detection, and characterization of these particles within micro and nanoscale sensors.