TARGETED DISRUPTION OF MOUSE EGF RECEPTOR - EFFECT OF GENETIC BACKGROUND ON MUTANT PHENOTYPE
TARGETED DISRUPTION OF MOUSE EGF RECEPTOR - EFFECT OF GENETIC BACKGROUND ON MUTANT PHENOTYPE
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DOI：
10.1126/science.7618084
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发表时间：
1995-07-14
期刊：
影响因子：
56.9
通讯作者：
MAGNUSON, T
中科院分区：
文献类型：
--
作者：
THREADGILL, DW;DLUGOSZ, AA;MAGNUSON, T
利用基因靶向技术在表皮生长因子受体位点（Egfr）上建立一个空等位基因。表型依赖于遗传背景。在CF-1背景下，EGFR缺乏导致由于内细胞团变性导致的植入期死亡。在129/Sv的背景下，纯合突变体由于胎盘缺陷在妊娠中期死亡；在CD-1背景下，突变体存活长达3周，并表现出皮肤、肾脏、大脑、肝脏和胃肠道的异常。与EGFR缺乏相关的多种异常表明该受体参与了广泛的细胞活动。
Gene targeting was used to create a null allele at the epidermal growth factor receptor locus (Egfr). The phenotype was dependent on genetic background. EGFR deficiency on a CF-1 background resulted in peri-implantation death due to degeneration of the inner cell mass. On a 129/Sv background, homozygous mutants died at mid-gestation due to placental defects; on a CD-1 background, the mutants lived for up to 3 weeks and showed abnormalities in skin, kidney, brain, liver, and gastrointestinal tract. The multiple abnormalities associated with EGFR deficiency indicate that the receptor is involved in a wide range of cellular activities.