Miniaturized immunoassay microfluidic system with electrokinetic control.
Miniaturized immunoassay microfluidic system with electrokinetic control.
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DOI：
10.1016/j.bios.2005.09.019
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发表时间：
2006-04
影响因子：
12.6
通讯作者：
Q. Xiang;Guoqing Hu;Yali Gao;Dongqing Li
中科院分区：
文献类型：
--
作者：
Q. Xiang;Guoqing Hu;Yali Gao;Dongqing Li
本文介绍了一种便携式异种免疫分析系统。该系统以聚二甲基硅氧烷(PDMS)微流控芯片为免疫反应器，以小型可编程高压测序仪为电源和流量控制器，以激光-光纤荧光检测模块为信号阅读器。该免疫分析系统的运行是自动的。顺序试剂分配和洗涤过程由可编程程序定序器控制。试剂用量仅为12μ，L，检测时间仅为26min。该系统对大肠杆菌O157：H7细菌抗原的检出限为0.3 ng/μ，低于以往用荧光显微镜检测的检出限。
A portable heterogeneous immunoassay system is presented in this paper. It consists of a poly(dimethylsiloxane) (PDMS) based microfluidic chip as the immunoreactor, a miniaturized programmable high voltage sequencer as the power supply and the flow controller, and a laser-optical fiber fluorescence detection module as the signal reader. The operation of this immunoassay system is automatic. The sequential reagent dispensing and washing processes are controlled by the programmable sequencer. The reagent consumption was only 12μL and the assay time was only 26min. The detection limit for Escherichia coli O157:H7 bacterial antigen with this miniaturized system was 0.3ng/μL, lower than that obtained using fluorescence microscope in previous studies.