On the peptide nature of vitamin Bc conjugate from yeast.
On the peptide nature of vitamin Bc conjugate from yeast.
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酵母维生素 Bc 结合物的肽性质。
DOI：
10.1021/ja01211a515
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发表时间：
1946
影响因子：
15
通讯作者：
Calkins Dg
中科院分区：
文献类型：
--
作者：
Pfiffner Jj;Calkins Dg
SIRS：已有证据支持这样的观点，即维生素的结合物由维生素与紫外线透明的含氮部分组成。1分子中非维生素BE部分由6个L(+)-谷氨酸分子组成。经18%的盐酸在100℃下水解16小时后，总氮的59.6%反应为-氨基酸N，2经微生物分析为谷氨酸氮。3·4，从298 mg.共轭甲酯220毫克。[A]24D+25.4(5.1%1N盐酸溶液)；C，32.75；H，5.6；N，7.8，7.6。标度：C，32.7；H，5.5；N，7.6。在比较的水解条件下，通过微生物测定，维生素总氮的20.1%以氨基酸态氮和谷氨酸态氮的形式存在。
Sirs: Evidence has been presented to support the view that vitamin Be conjugate consists of vitamin Be linked to an ultraviolet-transparent nitro-geneous moiety. 1 The non-vitamin Be portion of the moleculehas been found to consist of six molecules of l (+)-glutamic acid in peptide link-age. Following hydrolysis (18% hydrochloric acid for sixteen hours at 100) 59.6% of the total N re-acted as «-amino acid N, 2 which was accounted for as glutamic acid nitrogen by microbiological as-say. 3· 4 From 298 mg. of conjugate methyl ester 220 mg. of l (+)-glutamic acid hydrochloride was isolated.[a] 24D+ 25.4 (5.1% solution in 1 N hydrochloric acid; C, 32.75; H, 5.6; N, 7.8, 7.6. Caled.: C, 32.7; H, 5.5; N, 7.6. Under com-parable hydrolytic conditions 20.1% of the total nitrogen of vitamin Be reacted as-amino acid nitrogen and as glutamic acid nitrogen by microbiological assay.