Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations:: generating neurons from human skin?
Towards the development of a pragmatic technique for isolating and differentiating nestin-positive cells from human scalp skin into neuronal and glial cell populations:: generating neurons from human skin?
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DOI：
10.1111/j.1600-0625.2006.00471.x
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发表时间：
2006-10-01
影响因子：
3.6
通讯作者：
Paus, Ralf
中科院分区：
文献类型：
--
作者：
Kruse, Charli;Bodo, Enikoe;Paus, Ralf
小鼠皮肤的巢蛋白+毛囊相关细胞可以在体外分离并分化为神经元和神经胶质细胞。因此，我们一直在问人类皮肤是否也含有巢蛋白+细胞，以及这些细胞是否可以在体外分化为神经元和/或神经胶质细胞群。在这种方法学的试点研究中，我们表明，这两种情况确实是-故意只采用非常简单的技术，用于分离，繁殖和分化巢蛋白+细胞从正常人头皮皮肤及其附件，不需要选择性显微切割和组织隔室分离细胞培养之前。我们表明，原则上，可以维持和繁殖人皮肤巢蛋白+细胞以延长传代次数，并将其分化为神经元（即神经丝+和/或PGP9.5+）和神经胶质（即GFAP+，MBP+和/或O 4+）细胞群。因此，人类头皮皮肤可以作为一个高度可及的，丰富的，和方便的来源，为自体成体干细胞样细胞，提供自己被开发用于神经再生医学的目的。
Nestin+ hair follicle-associated cells of murine skin can be isolated and differentiated in vitro into neuronal and glial cells. Therefore, we have asked whether human skin also contains nestin+ cells, and whether these can be differentiated in vitro into neuronal and/or glial cell populations. In this methodological pilot study, we show that both are indeed the case - employing purposely only very simple techniques for isolating, propagating, and differentiating nestin+ cells from normal human scalp skin and its appendages that do not require selective microdissection and tissue compartment isolation prior to cell culture. We show that, it is in principle, possible to maintain and propagate human skin nestin+ cells for extended passage numbers and to differentiate them into both neuronal (i.e. neurofilament+ and/or PGP9.5+) and glial (i.e. GFAP+, MBP+ and/or O4+) cell populations. Therefore, human scalp skin can serve as a highly accessible, abundant, and convenient source for autologous adult stem cell-like cells that offer themselves to be exploited for neuroregenerative medicine purposes.