Paranoid potato Phytophthora-resistant genotype shows constitutively activated defense
Paranoid potato Phytophthora-resistant genotype shows constitutively activated defense
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DOI：
10.4161/psb.19149
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发表时间：
2012-01-01
影响因子：
2.9
通讯作者：
Andreasson, Erik
中科院分区：
文献类型：
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作者：
Ali, Ashfaq;Moushib, Laith Ibrahim;Andreasson, Erik
疫霉是薯蓣属植物最具破坏性的病原菌。需要具有不同作用模式的耐药源来对抗这种病原体的快速进化。为了更好地了解马铃薯对病原菌的防御机制，我们对几个马铃薯无性系进行了分析。在田间试验、全株试验和离体叶片试验中，两种基因型（Sarpo Mira和SW93-1015）对病原菌表现出较强的抗性。耐药基因型产生不同大小的过敏反应（HR）相关病变。SW93-1015的HR病变局限于非常小的区域，而Sarpo Mira的HR病变与经典抗性基因的主要来源——冬茄（Solanum desmissum）相似。SW93-1015可以被描述为cpr （PR基因的组成表达者）基因型，没有自发的显微镜或宏观HR病变。这可以通过组成型过氧化氢（H2O2）的产生和PR1（致病相关蛋白1）的分泌来证明。SW93-1015是最早被发现的具有经典的基于蛋白质的诱导防御的组成性表达的植物之一，没有任何明显的代谢成本或自发的细胞死亡损伤。
Phytophthora is the most devastating pathogen of dicot plants. There is a need for resistance sources with different modes of action to counteract the fast evolution of this pathogen. In order to better understand mechanisms of defense against P. infestans, we analyzed several clones of potato. Two of the genotypes tested, Sarpo Mira and SW93-1015, exhibited strong resistance against P. infestans in field trials, whole plant assays and detached leaf assays. The resistant genotypes developed different sizes of hypersensitive response (HR)-related lesions. HR lesions in SW93-1015 were restricted to very small areas, whereas those in Sarpo Mira were similar to those in Solanum demissum, the main source of classical resistance genes. SW93-1015 can be characterized as a cpr (constitutive expressor of PR genes) genotype without spontaneous microscopic or macroscopic HR lesions. This is indicated by constitutive hydrogen peroxide (H2O2) production and PR1 (pathogenesis-related protein 1) secretion. SW93-1015 is one of the first plants identified as having classical protein-based induced defense expressed constitutively without any obvious metabolic costs or spontaneous cell death lesions.