Diversity of strains of Salmonella enterica serotype enteritidis from English poultry farms assessed by multiple genetic fingerprinting
Diversity of strains of Salmonella enterica serotype enteritidis from English poultry farms assessed by multiple genetic fingerprinting
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DOI：
10.1128/jcm.39.1.154-161.2001
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发表时间：
2001-01-01
影响因子：
9.4
通讯作者：
Woodward, MJ
中科院分区：
文献类型：
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作者：
Liebana, E;Garcia-Migura, L;Woodward, MJ
需要可靠和充分区分的方法来区分表型水平以外的肠道沙门氏菌血清型肠道沙门氏菌的个体菌株;然而，关于哪种分子方法最适合于此目的尚未达成共识。此外，缺乏关于英国家禽环境中肠杆菌血清型分子指纹图谱的数据。本研究评估了经典方法（噬菌体分型）与三种成熟的分子方法（核糖体分型，基因组DNA的宏限制性分析和质粒分析）在104个分离株的多样性评估在英格兰的8个独立的家禽养殖场的肠球菌血清型的结合使用。鉴定多态性的最敏感的技术是PstI-SphII核糖体分型，区分共22种模式，其中10种被发现之间的噬菌体4型分离株。XhaI消化的基因组DNA的脉冲场凝胶电泳分离的分离株成只有6种类型，它们之间的微小差异。此外，在该人群中发现了14个质粒图谱。当所有的分型方法相结合，54种类型的菌株被区分，大多数家禽养殖场提出了各种菌株，这表明代表不同基因组的血清型肠道菌群在英国流通。总之，地理和动物来源的沙门氏菌血清型肠球菌分离株可能有相当大的影响，选择最佳的分型策略，个别程序，并不能依赖于一个单一的方法来区分菌株。
Reliable and sufficiently discriminative methods are needed for differentiating individual strains of Salmonella enterica serotype Enteritidis beyond the phenotypic level; however, a consensus has not been reached as to which molecular method is best suited for this purpose. In addition, data are lacking on the molecular fingerprinting of serotype Enteritidis from poultry environments in the United Kingdom. This study evaluated the combined use of classical methods (phage typing) with three well-established molecular methods (ribotyping, macrorestriction analysis of genomic DNA, and plasmid profiling) in the assessment of diversity within 104 isolates of serotype Enteritidis from eight unaffiliated poultry farms in England. The most sensitive technique for identifying polymorphism was PstI-SphII ribotyping, distinguishing a total of 22 patterns, 10 of which were found among phage type 4 isolates. Pulsed-field gel electrophoresis of XhaI-digested genomic DNA segregated the isolates into only six types with minor differences between them. In addition, 14 plasmid profiles were found among this population. When all of the typing methods were combined, 54 types of strains were differentiated, and most of the poultry farms presented a variety of strains, which suggests that serotype Enteritidis organisms representing different genomic groups are circulating in England. In conclusion, geographical and animal origins of Salmonella serotype Enteritidis isolates may have a considerable influence on selecting the best typing strategy for individual programs, and a single method cannot be relied on for discriminating between strains.