Primary structure of human alpha 2-antiplasmin, a serine protease inhibitor (serpin).
Primary structure of human alpha 2-antiplasmin, a serine protease inhibitor (serpin).
复制标题
人 α2-抗纤溶酶（一种丝氨酸蛋白酶抑制剂 (serpin)）的一级结构。
DOI：
10.1016/s0021-9258(19)75687-7
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
D. Collen
中科院分区：
文献类型：
--
作者：
W. Holmes;L. Nelles;H. Lijnen;D. Collen
血浆蛋白α 2-抗纤溶酶是丝氨酸蛋白酶纤溶酶的主要生理抑制剂，负责溶解纤维蛋白凝块。我们已经确定了成熟的人α 2-抗纤溶酶的一级结构的重叠cDNA片段制备从人肝mRNA的DNA测序。cDNA克隆通过与从α 2-抗纤溶酶的16-氨基酸肽序列推导的48-碱基对脱氧寡核苷酸探针杂交来鉴定。成熟的人α 2-抗纤溶酶含有452个氨基酸。它与属于丝氨酸蛋白酶抑制剂（serpin）超家族的其他五种蛋白质同源（23-28%）。其反应位点，即通过与其主要靶酶纤溶酶反应而裂解的肽键，由Arg 364-Met 365组成。该二肽对应于原型丝氨酸蛋白酶抑制剂α 1-抗胰蛋白酶的反应位点Met 358-Ser 359。
The plasma protein alpha 2-antiplasmin is the main physiological inhibitor of the serine protease plasmin, which is responsible for the dissolution of fibrin clots. We have determined the primary structure of mature human alpha 2-antiplasmin by DNA sequencing of overlapping cDNA fragments prepared from human liver mRNA. cDNA clones were identified by hybridization with a 48-base pair deoxyoligonucleotide probe deduced from the sequence of a 16-amino acid peptide of alpha 2-antiplasmin. Mature human alpha 2-antiplasmin contains 452 amino acids. It is homologous (23-28%) with five other proteins belonging to the serine protease inhibitor (serpin) superfamily. Its reactive site, i.e. the peptide bond cleaved by reaction with its primary target enzyme, plasmin, consists of Arg364-Met365. This dipeptide corresponds to the reactive site Met358-Ser359 of the archetypal serpin, alpha 1-antitrypsin.