Characterisation of the Virulence Factors and Genetic Types of Methicillin Susceptible Staphylococcus aureus from Patients and Healthy Individuals
Characterisation of the Virulence Factors and Genetic Types of Methicillin Susceptible Staphylococcus aureus from Patients and Healthy Individuals
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DOI：
10.1007/s12088-012-0286-7
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发表时间：
2012-12-01
影响因子：
3
通讯作者：
Thong, Kwai-Lin
中科院分区：
文献类型：
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作者：
Lim, King-Ting;Abu Hanifah, Yasmin;Thong, Kwai-Lin
甲氧西林敏感金黄色葡萄球菌是一种与医院和社区获得性感染相关的重要细菌病原体，可导致心内膜炎、皮肤组织感染和肺炎。本研究旨在了解甲氧西林敏感金黄色葡萄球菌(MSSA)的遗传特征，以及自2009年起从澳门大学医学院和健康学生中分离的金黄色葡萄球菌产生毒力因子的情况。在429份鼻拭子样本中，有67份是MSSA。用聚合酶链式反应(PCR)检测67株马来西亚临床分离株和社区分离株中21种不同毒力基因的流行情况，并用CoA基因的PCR-RFLP、agR分型、spA分型和PFGE分析其遗传特征。5个主要毒力基因分别为icA(79%)、efb和fnbA(61%)、sdrE(57%)和hlg(45%)。毒素基因(肠毒素、ETD和PVL)在临床菌株中显著高于社区菌株(P<；0.05)。检测到3种AGR基因：AGR I型(45%)、AGR III型(25%)和AGR II型(19%)。对67株MSSA进行CoA、55个脉冲型和21个SpA型的PCR-RFLP分析，共分出26个谱型。观察到4种新的spa类型(t7312、t7581、t7582和t7583)。综上所述，在马来西亚的MSSA菌株中观察到了不同的毒力谱，那里的毒素基因在临床菌株中更普遍。DNA图谱(CoA-RFLP、PFGE和SpA)与毒力类型之间没有相关性。来自临床和社区来源的马来西亚MSSA菌株具有遗传多样性和异质性。
Methicillin sensitive Staphylococcus aureus is an important bacterial pathogen associated with hospital- and community-acquired infections leading to endocarditis, skin tissue infection and pneumonia. The objective of this study was to determine both the genetic characteristics of methicillin-sensitive S. aureus (MSSA) strains, and the occurrence of virulence factors produced by S. aureus strains isolated from UMMC and healthy students in the University from year 2009. Out of 429 nasal swab samples, 67 were MSSA. The prevalence of 21 different virulence genes among 67 Malaysian clinical and community MSSA strains was determined by PCR, and their genetic features were assessed by PCR-RFLP of coa gene, agr types, spa typing and PFGE. The five predominant virulence genes were ica (79 %), efb and fnbA (61 % each), sdrE (57 %) and hlg (45 %). Toxin genes (enterotoxin, etd and pvl) were significantly more common (P < 0.05) in clinical strains compared to community strains. Three agr genotypes were observed: agr type I (45 %), agr type III (25 %) and agr type II (19 %). All 67 MSSA strains were distinguished into 26 profiles by PCR-RFLP of coa, 55 pulsotypes and 21 spa types. Four novel spa types (t7312, t7581, t7582 and t7583) were observed. In conclusion, different virulence profiles were observed in MSSA strains in Malaysia where toxin genes were more prevalent among clinical strains. No correlation between DNA profiles (coa-RFLP, PFGE and spa) and virulotypes was observed. The Malaysian MSSA strains from clinical and community sources were genetically diverse and heterogeneous.