Interaction of sulfated polysaccharides with intestinal Bacteroidales plays an important role in its biological activities
Interaction of sulfated polysaccharides with intestinal Bacteroidales plays an important role in its biological activities
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硫酸多糖与肠道拟杆菌的相互作用对其生物活性起着重要作用
DOI：
10.1016/j.ijbiomac.2020.12.024
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发表时间：
2021-01-31
影响因子：
8.2
通讯作者：
Ai, Chunqing
中科院分区：
文献类型：
--
作者：
Sun, Xiaona;Liu, Yili;Ai, Chunqing
硫酸多糖的生物活性已被证明与肠道微生物群有关，但其潜在机制仍不清楚。本研究通过体外发酵模型分析了太平洋鲍鱼硫酸多糖（AGSP）对人体肠道菌群的影响。结果表明，AGSP改变了肠道微生物群的整体结构，并增加了一些拟杆菌目成员的相对丰度，这意味着肠道拟杆菌目可以在AGSP的生物活性中发挥重要作用。为了阐明AGSP的作用机制，本研究从拟杆菌目拟杆菌属和副拟杆菌属中分离出部分菌株，并分析了它们对AGSP的利用特性。结果表明，肠道拟杆菌目对AGSP的利用具有种间依赖性，某些释放AGSP分解产物的种类促进了其他不能在AGSP中生存的种类的生长，形成了一个AGSP利用网络。体外细胞模型显示AGSP寡糖具有较好的抗炎活性和较弱的细胞毒性，说明AGSP的微生物降解会影响其与宿主细胞的反应。这些结果表明，多糖和肠道微生物之间的相互作用可以共同决定多糖对宿主健康的有益作用。(c)2020爱思唯尔B.V.保留所有权利。
The bioactivities of sulfated polysaccharides have shown to be associated with the gut microbiota, but the underlying mechanisms remain unclear. In this study, the effect of sulfated polysaccharides from pacific abalone (AGSP) on the human gut microbiota was analyzed via an in vitro fermentation model. The results revealed that AGSP altered the overall structure of the gut microbiota and increased relative abundances of some Bacteroidales members, implying that intestinal Bacteroidales can play important roles in the bioactivities of AGSP. To elucidate the underlying mechanisms, some species from the Bacteroides and Parabacteroides within Bacteroidales were isolated, and their characteristics on AGSP utilization were analyzed. It showed that AGSP utilization by intestinal Bacteroidales was species-dependent, and some species that liberated AGSP breakdown products promoted the growth of others unable to live in AGSP, forming an AGSP utilization network. The in vitro cell model showed that AGSP oligosaccharides had better anti-inflammatory activity and weaker cytotoxicity, implying that microbial degradation of AGSP can influence its reaction with host cells. These results indicated that the interaction between polysaccharides and gut microbes can together determine the beneficial effects of polysaccharides on the host health. (c) 2020 Elsevier B.V. All rights reserved.