Evaluation of the Oxidative Effect of Long-Term Repetitive Hyperbaric Oxygen Exposures on Different Brain Regions of Rats
Evaluation of the Oxidative Effect of Long-Term Repetitive Hyperbaric Oxygen Exposures on Different Brain Regions of Rats
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DOI：
10.1100/2012/849183
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Yildiz, Senol
中科院分区：
文献类型：
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作者：
Simsek, Kemal;Ozler, Mehmet;Yildiz, Senol
高压氧（HBO 2）暴露会影响氧化和抗氧化系统。这种效应与暴露时间和治疗持续时间呈正相关。本研究的目的是启发与氧化/抗氧化系统时，在大鼠脑组织中的长期和重复的方式管理的HBO 2的关系。将60只大鼠分为6个实验组（n = 8）和1个对照组（n = 12）。研究组大鼠每天暴露于2.8 ATA的90分钟HBO 2，持续5、10、15、20、30和40天。最后一次治疗后一天，处死动物;收获其整个脑组织并解剖成三个不同区域，即外部灰质（皮质）、内部白色物质和小脑。在这些组织中的脂质过氧化和蛋白质氧化水平和超氧化物歧化酶和谷胱甘肽过氧化物酶的活性进行了测定。丙二醛，羰基化蛋白和谷胱甘肽过氧化物酶水平被发现在不同的时间点在大脑皮层，内部白色物质和小脑，分别无显着增加。这些可比较的结果提供了证据，证明HBO治疗的安全性和/或至少在大鼠脑组织中成功的适应机制，即使在较长时间内施用也是如此。
Hyperbaric oxygen (HBO2) exposure affects both oxidative and antioxidant systems. This effect is positively correlated with the exposure time and duration of the treatment. The present study aims enlightening the relation of HBO2 with oxidative/antioxidant systems when administered in a prolonged and repetitive manner in brain tissues of rats. Sixty rats were divided into 6 study (n = 8 for each) and 1 control (n = 12) group. Rats in the study groups were daily exposed 90-min HBO2 sessions at 2.8 ATA for 5, 10, 15, 20, 30 and 40 days. One day after the last session, animals were sacrificed; their whole brain tissue was harvested and dissected into three different regions as the outer grey matter (cortex), the inner white matter and cerebellum. Levels of lipid peroxidation and protein oxidation and activities of superoxide dismutase and glutathione peroxidase were measured in these tissues. Malondialdehyde, carbonylated protein and glutathione peroxidase levels were found to be insignificantly increased at different time-points in the cerebral cortex, inner white matter and cerebellum, respectively. These comparable results provide evidence for the safety of HBO treatments and/or successful adaptive mechanisms at least in the brain tissue of rats, even when administered for longer periods.