A TWO-DIMENSIONAL NUCLEAR OVERHAUSER ENHANCEMENT (2D NOE) EXPERIMENT FOR THE ELUCIDATION OF COMPLETE PROTON-PROTON CROSS-RELAXATION NETWORKS IN BIOLOGICAL MACROMOLECULES
A TWO-DIMENSIONAL NUCLEAR OVERHAUSER ENHANCEMENT (2D NOE) EXPERIMENT FOR THE ELUCIDATION OF COMPLETE PROTON-PROTON CROSS-RELAXATION NETWORKS IN BIOLOGICAL MACROMOLECULES
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DOI：
10.1016/0006-291x(80)90695-6
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发表时间：
1980-01-01
影响因子：
3.1
通讯作者：
WUTHRICH, K
中科院分区：
文献类型：
--
作者：
KUMAR, A;ERNST, RR;WUTHRICH, K
最近开发的二维（2D）交叉弛豫光谱技术用于系统测量生物大分子的高分辨率1H NMR光谱中的选择性核奥弗豪泽增强（NOE）[例如，蛋白质]溶液中。与传统的一维NOE研究相比，二维NOE实验的主要优点是避免了在拥挤的光谱区域中预照射的有限选择性所产生的不利影响。它产生，与一个单一的仪器设置，一个完整的网络之间的所有质子的NOE的大分子。由此得到的分子内质子-质子距离的信息为研究生物大分子的空间结构提供了新的途径。
The recently developed technique of 2-dimensional (2D) cross-relaxation spectroscopy is utilized for systematic measurements of selective nuclear Overhauser enhancements (NOE) in the high resolution 1H NMR spectra of biological macromolecules [e.g., proteins] in solution. Compared to conventional 1-dimensional NOE studies, the 2D NOE experiment has the principal advantage that it avoids detrimental effects arising from the limited selectivity of preirradiation in crowded spectral regions. It yields, with a single instrument setting, a complete network of NOE between all the protons in the macromolecule. The resulting information on intramolecular proton-proton distances provides a new avenue for studies of the spatial structures of biopolymers.