Controlling the folding efficiency of an integral membrane protein
Controlling the folding efficiency of an integral membrane protein
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DOI：
10.1016/j.jmb.2004.07.041
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发表时间：
2004-09-24
影响因子：
5.6
通讯作者：
Booth, PJ
中科院分区：
文献类型：
--
作者：
Allen, SJ;Curran, AR;Booth, PJ
对完整膜蛋白折叠机制的研究远远落后于对水溶蛋白的研究，因为蛋白质折叠这个术语与水溶蛋白是同义词。疏水性膜蛋白，尤其是那些具有跨膜α-螺旋基序的蛋白，经常被认为太难处理了。我们发现，储存的曲率弹性应力可以用来指导这些完整的膜蛋白折叠系统的设计。合成磷脂酰胆碱/磷脂酰乙醇胺类脂双分子膜的曲率弹性应力可以用来控制折叠速度和折叠蛋白质的产量。物理双层性质的使用使这一方法超越了所涉及的脂类的特定化学成分。(C)2004爱思唯尔有限公司。保留所有权利。
Research into the folding mechanisms of integral membrane proteins lags far behind that of water-soluble proteins, to the extent that the term protein folding is synonymous with water-soluble proteins. Hydrophobic membrane proteins, and particularly those with transmembrane a-helical motifs, are frequently considered too difficult to work with. We show that the stored curvature elastic stress of lipid bilayers can be used to guide the design of efficient folding systems for these integral membrane proteins. The curvature elastic stress of synthetic phosphatidylcholine/phosphatidylethanolamine lipid bilayers can be used to control both the rate of folding and the yield of folded protein. The use of a physical bilayer property generalises this approach beyond the particular chemistry of the lipids involved. (C) 2004 Elsevier Ltd. All rights reserved.