ANALYSIS OF GLYCOFORM OF O-GLYCAN FROM HUMAN MYELOMA IMMUNOGLOBULIN-A1 BY GAS-PHASE HYDRAZINOLYSIS FOLLOWING PYRIDYLAMINATION OF OLIGOSACCHARIDES
ANALYSIS OF GLYCOFORM OF O-GLYCAN FROM HUMAN MYELOMA IMMUNOGLOBULIN-A1 BY GAS-PHASE HYDRAZINOLYSIS FOLLOWING PYRIDYLAMINATION OF OLIGOSACCHARIDES
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DOI：
10.1016/s0003-2697(05)80034-x
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发表时间：
1992-10-01
影响因子：
2.9
通讯作者：
KOBAYASHI, Y
中科院分区：
文献类型：
--
作者：
IWASE, H;ISHIIKARAKASA, I;KOBAYASHI, Y
A comparative study was made on the glycoform ofO-glycan from human myeloma immunoglobulin A1. By gas-phase hydrazinolysis, O-glycan was released from its hinge portion. The released oligosaccharide was pyridylaminated and separated by a two-dimensional analytical method of gel filtration and reverse-phase HPLC. Four major pyridylamino derivatives (P1–P4) were obtained. The neutral component (P4) among them was identified as Ga1β1,3GalNAc-PA by cochromatography with an authentic standard pyridylamino sugar. The desialylation of the other components indicated the largest P1 and middle size P2 components possibly corresponded to a disialylated structure, NeuAcα2,3Galβ1,3(NeuAcα2,6)GalNAc-PA, and a monosialylated component, NeuAcα2,3Galβ1,3Ga1NAc-PA,respectively. The structural assignment of P3 is still incomplete. Four similar components were also detected in bovine fetuin whose relative content (P1:P2: P3:P4) was 16:43:19:22. The relative content (%) of P1–P4 (glycoform) in IgAl from the healthy control waauthor0.1±3.3,48.2±4.6,7.0±2.6,and 34.7±4.5. The glycoform of O-glycan on IgA1 thus appears the same for any individual. Analysis of IgA1 myeloma protein indicated glycoforms distinct from those of the healthy controls. The relative content of these component could be classed as 2:8:0:90 (Type I, only one case designated as Kita), 5:24:3:68 (Type II, seven cases), and 9:41:5:45 (Type III, four cases). Thus, the results for IgAl myeloma protein indicate that at least three glycoforms of O-glycan are possible for the IgA1 hinge structure. However, only one glycoform was found in the healthy controls.
